虎纹蛙舌肥大细胞组化及免疫组化  

Studies on the Mast Cells in the Tongue of Frog(Rana tigrina rugulosa) by Histochemical and Immunohistochemical Method

作　　者：林旋[1] 王寿昆[1] 林树根[1] 陈梅芳[1] 李玲玲[1] 

机构地区：[1]福建农林大学动物科学学院/福建省动物药物工程实验室,福州350002

出　　处：《吉林农业科学》2014年第6期81-84,共4页Journal of　Jilin Agricultural Sciences

基　　金：福建省教育厅项目(JA11092)

摘　　要：应用改良甲苯胺蓝(MTB)、阿尔新蓝-藏红O(AB/SO)、甲基绿-派洛宁(MG-P)、天青Ⅱ-伊红-瑞氏混合液等4种组化染色技术进行染色,并用小鼠抗人肥大细胞类胰蛋白酶单克隆抗体AAl通过ElivisionTM免疫组化染色方法,对虎纹蛙舌头肥大细胞进行染色。结果表明:虎纹蛙舌头中的肥大细胞,大小形态不一。虎纹蛙舌组织中分布有大量肥大细胞,主要分布于固有层的结缔组织中和舌头腺体之间,肌层也可见到大量的肥大细胞,并且有沿血管分布的特点。在用ElivisionTM免疫组化染色方法中,单克隆抗体AAl与虎纹蛙舌头组织中肥大细胞中的类胰蛋白酶有良好的交叉反应。类胰蛋白酶阳性细胞数量很少,分布于舌组织固有层和腺体之间。而人胃癌间质中可见多量类胰蛋白酶阳性细胞。The mast cells of the back, abdominal skin and tongue of the frog, Rana tigrina rugulosa, were stained using modified toluidine blue （MTB）, Alcian blue Safranin O （AB/SO）, methyl green-Pyronine （MG-P）, Azure Ⅱ- Eosin-Wright mixture and a murine monoclonal antibody （AA1） that raised against human mast cell tryptase by ElivisionTM immunohistochemical. The results showed that the mast cells in the tongue of the frog had different size and form. There were lots of mast ceils in the tongue tissue of the frog which were mainly distributed between the connective tissue of lamina propria and lingual gland. Lots distributed in muscular layer and intended to be around blood vessels. With ElivisionTM immunohistochemical staining method, the monoclonal antibody AA1 and the tryptase of the mast cells in the tongue tissue of the frog had an excellent cross-reaction. Small amount of the positive tryptase cells were found in the frog and mostly distributed between the lamina propria and gland of the tongue tissue. There were lots of tryptase-positive mast cell in the mesenchyme of the human gastric cancer.

关 键 词：肥大细胞 虎纹蛙 异质性 类胰蛋白酶 组化性质 

分 类 号：Q952[生物学—动物学]