二仙汤对去卵巢骨质疏松模型大鼠血清I型胶原氨基端延长肽影响的实验研究  

Impact of Guilu Erxian Glue on Serum Type Ⅰ Collagen Amino-terminal Extension Peptide in Ovariectomized Rat Model of Osteoporosis

作　　者：张瑶[1] 朱旭[1] 刘菊[1] 马威[1] 

机构地区：[1]湖北省武汉市第一医院,武汉市430030

出　　处：《广西医学》2014年第11期1593-1595,共3页Guangxi Medical Journal

摘　　要：目的观察二仙汤对去卵巢骨质疏松模型大鼠骨质疏松的干预作用。方法取3月龄SPF级健康雌性SD大鼠50只,随机分为假手术组、模型组、治疗组和西药对照组、中药对照组,每组10只。假手术组行假手术,其余4组行双侧卵巢摘除术,术后4周开始治疗。治疗组灌胃二仙汤[按10 g/(kg·d)剂量],中药对照组灌胃仙灵骨葆[按10 g/(kg·d)剂量],西药对照组灌胃戊酸雌二醇片[按0.2 mg/(kg·d)剂量],假手术组及模型组灌胃生理盐水(按1 ml/100 g体重)。给药12周后以双能X线骨密度测定仪测定骨密度,麻醉后采血,分离血清后测定各组动物I型胶原羧基端肽(β-Crosslaps)、总I型胶原氨基端延长肽(P1NP)、Ca和P的含量。结果与模型组比较,假手术组、治疗组、中药对照组、西药对照组Ca/P、骨密度、P1NP比值均显著增高(P<0.05),β-Crosslaps含量均显著降低(P<0.01);治疗组、中药对照组、西药对照组P含量显著降低(P<0.05);治疗组Ca含量显著高于中药对照组及西药对照组(P<0.01),西药对照组β-Crosslaps含量降低(P<0.01)。治疗组及西药对照组骨密度低于假手术组(P<0.01),3治疗组之间P1NP含量无差异(P>0.05)。结论二仙汤对去卵巢大鼠骨质疏松的形成有干预作用。Objective To observe the effect of Guilu Erxian glue on ovariectomized rat model of osteoporosis.Methods Fifty SPF healthy female SD 3-month-old rats were randomly divided into sham operation group,model group,treatment group,western medicine control group and traditional Chinese medicine control group. The rats in the sham operation group underwent sham surgery,and the other four groups underwent bilateral oophorectomy. We started the treatments 4 weeks after surgery. The rats were given Guilu Erxian glue by gavage in the treatment group[at a dose of 10 g /（ kg·d） ],Xianlinggubao in traditional Chinese medicine control group[at a dose of 10 g /（ kg·d） ],estradiol valerate tablets in western medicine control group [at a dose of 0. 2 mg /（ kg·d） ],respectively. The rats in the sham operation group and model group were given normal saline by gavage（ 1 ml /100 g by weight）. The bone density of rats was measured using dual-energy X-ray absorptiometry 12 weeks after administration,and the blood samples were collected after anesthesia. Type I collagen carboxy-terminal peptide（ β-Crosslaps）,the total extension of type I collagen amino terminal peptide（ P1NP）,and the contents of Ca,P in serum of rats were measured in each group after administration.Results Compared with model group,serum Ca / P,bone density and P1 NP ratio of animals increased significantly in the sham operation group,treatment group,traditional Chinese medicine control group and western medicine control group（ P〈0. 05）,and the β-Crosslaps content of animals decreased significantly（ P〈0. 01）. The serum P content of animals decreased significantly in the treatment group,traditional Chinese medicine control group and western medicine control group（ P〈0. 05） compared with model group（ P〈0. 05）. The serum Ca content of animals was significantly higher in the treatment group in contrast with those in the Chinese medicine control group and western medicine control group（ P〈0. 01）,and the β-Crosslaps c

关 键 词：骨质疏松 双侧卵巢切除术 二仙汤 大鼠 骨密度 

分 类 号：R681[医药卫生—骨科学]