高通量测序技术在香蕉抗枯萎病研究中的应用  

Application of high-throughput sequencing technology in banana resistant to Fusarium wilt

作　　者：孙嘉曼[1] 韦弟[1,2] 覃柳燕[1,2] 魏源文[1] 郭文峰[1] 吕维莉[1] 张进忠[1,2] 卢江[1,3] 

机构地区：[1]广西作物遗传改良生物技术重点开放实验室,南宁530007 [2]广西农业科学院生物技术研究所,南宁530007 [3]中国农业大学食品与营养工程学院,北京100083

出　　处：《南方农业学报》2014年第11期1921-1925,共5页Journal of Southern Agriculture

基　　金：广西八桂学者专项资金项目([2013]3号)

摘　　要：文章综述了香蕉枯萎病的危害及防治、高通量测序技术的发展及其在香蕉抗病研究中的应用,发现香蕉受枯萎病菌侵染与JA信号途径有关,编码乙烯合成酶ACC氧化酶的基因和乙烯应答转录因子(ERF)在病原菌侵染香蕉后强烈表达。因此,今后可利用高通量测序技术对香蕉基因组进行研究,在香蕉基因组已经测序完成的基础上,通过转录组分析技术挖掘香蕉抗性突变体抗枯萎病的基因资源,获得香蕉抗性突变体抗Foc4的基因和位点;通过比较基因组学与生物信息学相结合,准确定位物种表型变异相关的基因组区域,加速香蕉中未知功能基因的挖掘和利用;克隆并研究控制重要抗性基因,掌握其抗病规律,为生产中防控香蕉枯萎病提供依据和指导,从而加速抗病品种的选育过程,获得抗性持久的新品种。With the recent development and application in agricultural research, the high-throughput sequencing technology offers new opportunities to speed up the breeding process for new banana variety improvement.This paper aimed to review the damage and control measures of banana Fusarium wilt, as well as the development of high-throughput sequencing technology and its application in banana resistant to diseases. At present, the related researchers have found that the Foc infection to banana was associated with the JA signaling pathways, and the genes encoding ethylene synthetase ACC oxidase and ethylene response transcription factor（ERF） could strongly express after pathogen infection to banana. In the future, high-throughput sequencing technologies should be applied to study the banana genome. On the basis of finishing banana genomic sequencing, the gene resources of banana resistance mutants against Fusarium wilt should be exploited by using transcriptome technologies to get genes and loci of banana resistant mutants against Foc4. The related genomic regions of species phenotypic variation should be mapped precisely by combining with comparative genomics and bioinformatics to accelerate the exploitation and application of unknown functional genes in banana. The important resistant control genes should be cloned to master its disease resistance law, which could provide references and indirections on controlling banana Fusarium wilt and accelerate resistant varieties breeding process for getting new varieties with lasting resistance.

关 键 词：高通量测序 香蕉枯萎病 病害防治 抗病机制 抗病育种 

分 类 号：S332.2[农业科学—作物遗传育种]