蛋白截留法评价超滤膜分离性能研究  

Discussion about assessment of ultrafitration membranes retention performance by seprating protein method

作　　者：罗鸣[1] 尚闽 郑祥[1] 

机构地区：[1]中国人民大学环境科学与工程系,北京100872

出　　处：《膜科学与技术》2014年第6期56-61,共6页Membrane Science and Technology

基　　金："十二五"国家科技支撑计划(2012BAJ19B02);教育部"新世纪优秀人才支持计划资助"(NCET-12-0531)

摘　　要：依据HY/T 050—1999《中空纤维超滤膜测试方法》标准方法,采用不同分子量蛋白的截留率对两种超滤膜的截留分子量进行测试,并考察测试过程中运行时间、初始浓度和操作压力等参数对膜分离性能测试结果的影响.研究结果表明,标准中规定的几种参数中,蛋白的初始浓度对膜性能测试的结果影响很大.当牛血清蛋白浓度由100 mg/L增加至1 000 mg/L时,蛋白的截留率从3.5%增加到80.7%;蛋白原液浓度越高,检测所得到的膜截留分子量数值越低,造成测试结果与实际膜分离性能的偏差越大,但过低的蛋白原液浓度也会影响测试结果的稳定性.因此,在测试过程中可适当降低初始浓度为100-500mg/L之间,适当延长运行时间到40min左右,以提高分离性能测试的准确性.Solute rentention method is widely used for determining the ultrafitration membrane separation performance.This study investigates the accuracy of MWCO（Molecular Weight Cut Off）determined by using different protein molecules.A series of experiments have been carried out to determine protein retention in various conditions（runtime,protein concentration and transmembrane pressure）during filtration through hollow fiber membrane and flat-sheet membrane.The results showed the BSA rejection rate increased as the initial concentration increasing,the retention rate changed from 3.5%to 80.7% when initial concentration increased 100 mg/L to 1000 mg/L.And the MWCO was inaccurate with high concentration of protein molecules.High concentration may cause error of the MWCO test,but low concentration may influence the stability of the test results.With adjusting the concentration to 100-500mg/L and the runtime to 40 min,the data of MWCO could be more reliable.

关 键 词：超滤膜 截留分子量 基准物质 

分 类 号：TQ028.8[化学工程]