去卵巢大鼠心脏结构和心肌细胞的改变  

The Changes of Cardiac Structure and Myocardial Cell in Ovariectomized Rats

作　　者：杨慕坤[1] 崔广霞[1] 陈醒[1] 席思思[1] 张婧[1] 王珂[1] 秦丽华[2] 白文佩[1] 

机构地区：[1]北京大学第一医院妇产科,100034 [2]北京大学医学部

出　　处：《国际妇产科学杂志》2014年第6期670-673,I0001,共5页Journal of International Obstetrics and Gynecology

基　　金：国家自然科学基金(81270666);2014年北京大学第一医院科研基金

摘　　要：目的:研究去卵巢大鼠心脏结构、功能以及心肌纤维化情况。方法:将20只Sprague-Dawley(SD)雌性大鼠随机分为假手术组(Sham组)和去卵巢组(OVX组),行超声心动图(UCG)检查,取心脏组织石蜡包埋,行HE及Masson染色。结果:与Sham组相比,OVX组左心室室间隔厚度明显增厚(P<0.05),心肌细胞面积与心肌细胞核面积显著增大(P<0.01),心脏胶原容积分数以及心脏血管周围胶原面积明显增多(P<0.05)。结论:去卵巢大鼠左心室室壁显著增厚,心肌细胞和间质胶原均出现重构改变。Objective：To investigate cardiac structure and function,myocardial cell morphology,and myocardial fibrosis in ovariectomized rats. Methods：Female Sprague-Dawley rats were divided into 2 groups , sham-operated group （Sham group） and ovariectomized group （OVX group）,each group has 10 rats. The OVX group underwent bilateral oophorectomy. The 2 groups have undergone UCG to evaluate cardiac structure and function. The heart tissue of each rats were taken HE and Masson staining ,to evaluate myocardial morphological changes. Results：Compared with the Sham group, left ventricular septal of OVX group was significantly thicker （P〉0.05）;OVX rats′ myocardial cells area increased significantly （P〉0.01）;ovariectomized rats′ cardiac collagen volume fraction and cardiac perivascular collagen area increased significantly （P〉0.05）. Conclusions：In ovariectomized rats,left ventricular septal and posterior wall have been significant thickening,reconstruction change appeared both in myocardial cells and interstitial collagen.

关 键 词：更年期 心内膜心肌纤维化症 心血管疾病 超声心动描记术 去卵巢大鼠 

分 类 号：R711.75[医药卫生—妇产科学]