脉冲电磁场协同多孔钛合金对兔长骨缺损修复效果的微观结构分析  

Microstructural Analysis on Repairing Effects of Synergetic Application of Pulsed Electromagnetic Fields and Porous Titanium Alloy on Rabbit Long Bone Defects

作　　者：景达[1] 佟世超[1] 翟明明[1] 李小康[2] 申广浩[1] 罗二平[1] 

机构地区：[1]第四军医大学生物医学工程系,西安710032 [2]第四军医大学西京医院骨科,西安710032

出　　处：《高电压技术》2014年第12期3793-3798,共6页High Voltage Engineering

基　　金：国家自然科学基金(81471806;31270889);陕西省自然科学基金(2014JQ4139)~~

摘　　要：多孔钛合金(p Ti)是目前较为理想的骨替代修复材料,但其在微观水平上仍难以与骨组织充分骨整合。为此,研究了脉冲电磁场(PEMF)协同p Ti作用对兔长骨缺损的修复效果。采用电子束熔融技术制备孔隙率70%的Ti6Al4V多孔钛合金圆棒;于12只成年兔股骨外侧髁构建骨缺损,填充以p Ti,并采用自行研制的基于"3-Helm Holz线圈"的电磁发生系统产生2 m T、15 Hz的PEMF进行实验。结果表明:与单独的p Ti作用相比,在PEMF和p Ti的协同作用下,6周后,p Ti中的骨体积百分比提高了78.5%,骨小梁数量提高了21.7%,骨小梁厚度增大了41.8%,骨小梁间隙减小了30.7%,骨表面积密度减少了36.2%。因此,PEMF显著提高了p Ti的骨缺损修复效率,PEMF协同p Ti作用是一种更为高效的长骨缺损修复方法。Porous titanium alloy（p Ti） is an ideal bone substitutes for bone defect repair, whereas the poor osseointegration of p Ti with bone tissues at the microstructural level is still remained an issue. We investigated the effect of repairing bone defect in rabbit long bones pulsed electromagnetic field（PEMF） combined with p Ti. Ti6Al4 V p Ti rods with porosity of 70% were fabricated using the electron beam melting technology. We filled p Ti implants into 12 rabbits with bone defect of femoral lateral condyle. Then we used a custom-designed electromagnetic fields generator based on ＇3-Helm Holz coils＇ to produce PEMF of 2 m T and 15 Hz and treat the rabbits. The results showed that, after six weeks, compared with independent p Ti application, the synergetic treatment of PEMF and p Ti significantly increased the bone volume fraction by 78.5%, the trabecular number by 21.7% and trabecular thickness by 41.8%, and it decreased the trabecular space and the bone surface/bone volume by 30.7% and 36.2%, respectively. Therefore, PEMF treatment significantly boosts the repair efficiency of p Ti on bone defects, and the synergetic application of PEMF and p Ti is an effective method for repairing long bone defect.

关 键 词：脉冲电磁场 多孔钛合金 骨缺损 骨小梁 微观结构 显微CT 

分 类 号：R683[医药卫生—骨科学] R318.08[医药卫生—外科学]