尾矿库周边地下水对SD大鼠的毒性效应研究  

Toxicology Study on Contaminated Groundwater Close to a Tailing Reservoir

作　　者：刘娟[1] 张雪峰[1] 司万童[2] 王建英[1,2] 贺小英[2] 吴飞[2] 

机构地区：[1]内蒙古科技大学能源与环境学院,内蒙古包头014010 [2]内蒙古科技大学数理与生物工程学院,内蒙古包头014010

出　　处：《环境科学与技术》2014年第12期129-133,165,共6页Environmental Science & Technology

基　　金：环保公益性行业科研专项(201309005)

摘　　要：金属冶选尾矿库渗漏对其周边区域地下水环境造成的复合污染是一个普通存在的问题。为了明确某尾矿库周边地下水污染对生物的影响状况,以该尾矿库周边地下水为实验用水,以SD大鼠为实验动物,进行尾矿库周边地下水污染对SD大鼠的毒性效应研究。实验中尾矿库周边地下水中污染物监测采用电感耦合等离子体质谱技术(ICP-MS)、分光光度法和滴定法;SD大鼠的饮食行为和体重测定采用称量法和统计法;SD大鼠肝、肾细胞DNA的损伤检测采用单细胞凝胶电泳法。结果显示,尾矿库周边地下水中的硫酸盐、硝酸盐氮、Zn等污染物均有不同程度的超标现象。尾矿库周边地下水污染对SD大鼠的生长性能有抑制作用。尾矿库周边地下水污染对SD大鼠肝、肾细胞的DNA损伤作用显著(p<0.05),受损程度随着与尾矿库距离的减少而增加;尾矿库周边地下水中硝酸盐氮、Zn污染与肝、肾细胞DNA损伤之间具有相关性(p<0.05)。结果表明尾矿库渗漏水对周边地下水造成了不同程度的污染,受污染的周边地下水对SD大鼠具有较强的毒性效应。This paper refers to groundwater pollution caused by leakage of a tailing reservoir in Inner Mongolia. To make clear the adverse impact on the biological sphere,the investigation was conducted for which the contaminated groundwater samples were taken ICP-MS and ion chromatography used for pollutants monitoring;meanwhile,SD rats were used as toxicological testing animals whose dietary behaviors were measured by weighing SD rats with statistical and gravimetric methods. In addition, DNA damages of SD rats＇ liver and kidney cell were diagnosed using the single-cell gel electrophoresis technique. The toxicology study revealed that contaminants such as nitrate nitrogen,Zn in the surrounding groundwater that had exceeded the tolerant limits of relevant drinking water standard were remarkably correlated to DNA damages of SD rats liver and kidney cell（p0.05）,with an inhibitory effect on the physiological properties of SD rats,and the damages were found increased in the groundwater that was close to the tailing reservoir. In conclusion,the leakage of the tailings reservoir has resulted in serious pollution of the surrounding groundwater.

关 键 词：尾矿库 地下水 SD大鼠 DNA损伤 

分 类 号：X503.22[环境科学与工程—环境工程]