两种游离DNA检测方法的比较研究  

Comparative study of two methods for detection of cell-free DNA

作　　者：施维[1] 戚菁[1] 申娴娟[1] 吴信华[1] 鞠少卿[1] 刘才旺[1] 

机构地区：[1]南通大学附属医院外科综合实验室,江苏226001

出　　处：《交通医学》2014年第6期579-580,585,共3页Medical Journal of Communications

基　　金：南通市科技项目(HS2016056);南通大学校级自然科研项目(11Z014)

摘　　要：目的:对比评价人血清游离DNA的分支DNA(b DNA)检测和实时荧光定量聚合酶链反应(RT-q PCR)检测方法。方法:收集44例人血清标本,分别运用b DNA技术和荧光定量PCR检测其游离DNA浓度,对2种方法检测结果做分析比较。同时采用b DNA技术分别检测其中10例血清和血浆游离DNA含量。结果:b DNA技术的检测结果和荧光定量PCR具有较高的相关性(r=0.7077,P<0.0001);血清较血浆具有较高的游离DNA的水平。结论:b DNA操作简便且结果可信,适用于人群大规模筛查游离DNA水平。Objective：Compare two methods to detect the concentrations of serum cell-free DNA by using branched DNA assay （bDNA） and real-time fluorescence quantitative real-time polymerase chain reaction （RT-qPCR）. Methods： 44 samples of serum cell-free DNA were measured by both bDNA based on Alu sequence and qPCR. The correlation between the two methods was analyzed by a Spearman rank test. Detection of cell-free DNA in human plasma and serum was ana_lyzed by bDNA. Results： It was demonstrated in the present study that the bDNA method was highly correlated with real-time PCR （r=0.7077, P〈0.0001）. Cell-free DNA concentrations were higher in serum than in plasma. Conclusion： bDNA assay had advantages of lower time and labor compared to the qPCR method that is applicable to screening consideration.

关 键 词：游离DNA 分支DNA 实时荧光定量聚合酶链反应 化学发光免疫分析仪 

分 类 号：Q523[生物学—生物化学]