甘肃产柴胡柴胡皂苷a含量测定及抗抑郁作用评价  

Determination of Saikosaponin-a in Radix bupleuri in Gansu Province and appraisement of its antidepressant activity

作　　者：宁艳梅[1] 杨秀娟[1] 杨韬[1] 

机构地区：[1]甘肃中医学院药学院,甘肃兰州730000

出　　处：《中兽医医药杂志》2014年第6期34-37,共4页Journal of Traditional Chinese Veterinary Medicine

基　　金：甘肃中医学院中青年科研基金项目(编号:BH2012-024)

摘　　要：目的:比较甘肃产柴胡不同炮制品中柴胡皂苷a的含量及其对小鼠的抗抑郁作用。方法:采用HPLC测定甘肃产柴胡不同炮制品中柴胡皂苷a含量,并通过自主活动、悬尾和强迫游泳实验及脑内5-HT及NE含量的测定,比较柴胡不同炮制品对行为绝望抑郁模型小鼠的影响。结果:甘肃产柴胡不同炮制品中柴胡皂苷a的含量以酒柴胡含量最高,鳖血柴胡次之。不同炮制品均能缩短两种行为绝望模型小鼠的不动时间,以鳖血柴胡及醋柴胡效果最明显;不同炮制品均可提高小鼠脑内5-HT含量,以鳖血柴胡效果最显著,具有显著性差异。结论:炮制可使甘肃产柴胡皂苷a含量发生变化,并改善小鼠的绝望行为,增加脑内5-HT含量而显示抗抑郁作用,有良好的开发前景。Objective： To determine saikosaponin-a in different processed products of Radix bupleuri in Gansu Province and study its antidepressant effect. Methods： HPLC was used to test the content of saikosaponin-a in different processed products of Radix bupleuri and the antidepressant effects were observed by using tile spontaneous activities test, tail suspension test. forced swimming test in mice, and 5-HT and NE in brain were detected and to be compared. Results： The content of saikosaponin-a in wine processing Radix bupleuri was the highest, then was soli-shelled turtle blood-baked ones. Different processed products could all reduce the immobility time of mice in two kinds of behavioral despair model, especially soft-shelled turtle bloodbaked ones and vinegar-baked ones. Different processed products could all increase the 5-HT level in mice brain, especially soft-shelled turtle blood-baked ones. Conclusion： Radix bupleuri in Gansu Province has promising developmenl prospect. Processing can change its saikosaponin-a content and enhance its antidepressant effect by improving behavioral despair of mice and increasing the 5-HT level in brain.

关 键 词：甘肃产柴胡 炮制品 柴胡皂苷A 行为绝望抑郁模型 小鼠 

分 类 号：S853.7[农业科学—临床兽医学]