二化螟幼虫病原真菌的分离纯化及其对烟粉虱和褐飞虱的毒力研究  

Study on the purification of pathogenic fungi from the rice stem borer larvae and the control of Bemisia tabaci and Nilaparvata lugens

作　　者：王艳秋[1] 洪勇[1] 岳霄霄[1] 林华峰[1] 

机构地区：[1]安徽农业大学植物保护学院,合肥230036

出　　处：《环境昆虫学报》2014年第6期943-950,共8页Journal of Environmental Entomology

基　　金：中国烟草总公司科研重点项目(110201202003);"十二五"农村领域国家科技计划(2011AA10A204);公益性行业(农业)科研专项(201303017)

摘　　要：通过对罹病二化螟Chilo suppressalis(Walker)幼虫病原真菌的分离纯化,筛选出5种不同真菌,探究不同营养对不同真菌生物活性的影响,并测定其对烟粉虱Bemisia tabaci(Gennadius)和褐飞虱Nilaparvata lugens(Stal)成虫和若虫的室内毒力。选择毒力最强的菌株,通过生物型的初步鉴定,明确高毒力菌株的种类。结果表明:所筛选的5种真菌对两种害虫均具有较高的毒力,其中菌株A26和Bbr14对烟粉虱和褐飞虱具有较强的毒力,在处理后第9 d,菌株A26对烟粉虱成虫和若虫的累计校正死亡率均最高分别达84.69%、77.62%,感染率为91.17%、88.24%,LT50为4.31 d、4.87 d;处理后第10 d对褐飞虱成虫和若虫的累计校正死亡率达87.54%、73.18%,感染率为90.13%、87.13%,LT50为4.27d、5.14 d,而菌株Bbr14的效果仅次于A26。鉴定的结果显示菌株A26为曲霉,菌株Bbr14为球孢白僵菌,曲霉的安全性有待研究,球孢白僵菌的应用技术已较成熟,有望能在防治这两类重要害虫中发挥作用。By the separation and purification of pathogenic fungi from Chilo suppressalis Walker larva, 5 different fungi were screened. The effect of different nutrition on the biological activity of fungi were explored and the toxicity to adults and nymphs of Bemisia tabaci and Nilaparvata lugens were measured. The most toxic strains were selected and the biotypes were clear through preliminary identification. The results showed that 5 species of fungi have high toxicity to the two insect pests, and the toxicity of A26 and Bbrl4 strains are stronger than others. After processing 9 days, the accumulative mortality of A26 strain on adults and nymphs of B. tabaci was the highest, 84.69%, 77.62% respectively, the infection rate was 91.17% , 88.24% and the LT50 was 4.31 d, 4.87 d, both were the lowest. After processing 10 days, the accumulative mortality of A26 strain on adults and nymphs of N. lugens was 87.54%, 73.18% , the infection rate was 90. 13%, 87.13% and the LT50 was 5.14 d. The effect of Bbrl4 strain was lower. Identification results showed that A26 strain was Aspergillus, Bbrl4 strain was Beauveria bassiana and the effects of different nutrition on the biological activity were difference. These provided the basis for the biological control of pests and fermentation.

关 键 词：病原真菌 生物特性 烟粉虱 褐飞虱 毒力测定 

分 类 号：Q965.9[生物学—昆虫学] S476[农业科学—农业昆虫与害虫防治]