嗜线虫致病杆菌HB310培养基筛选和培养条件优化  

Screening of original medium and optimizing fermentation conditions of Xenorhabdus nematophila HB310

作　　者：王永娟[1] 张杰[1] 孔繁芳[1] 南宫自艳[1] 宋萍[1] 王勤英[1] 

机构地区：[1]河北农业大学植物保护学院,河北保定071000

出　　处：《环境昆虫学报》2014年第6期997-1003,共7页Journal of Environmental Entomology

基　　金：河北省自然科学基金(C2014204117);河北省现代农业产业技术体系棉花创新团队

摘　　要：嗜线虫致病杆菌Xenorhabdus nematophila是与小卷蛾斯氏线虫Steinernema carpocapsae互惠共生的一种革兰氏阴性菌,对多种农业害虫都具有很高的杀虫活性。本研究对多种影响嗜线虫致病杆菌HB310菌株发酵培养的因素进行了筛选优化。通过单因素试验确定最佳碳源、氮源和无机盐分别为葡萄糖、牛肉蛋白胨和KH2PO4;通过正交实验确定培养基的最优组合为:葡萄糖2%、牛肉蛋白胨1%、牛肉浸膏0.3%和K2HPO41%;最佳摇瓶培养条件为:接种量6%、培养基p H 7.5、摇床转速200 rpm、培养温度28℃和培养时间48 h,在此条件下,嗜线虫致病杆菌HB310菌株生长速率最快,菌液的杀虫活性最高。Xenorhabdus nematophila, a Gram -negative bacterium, is associated symbiotically with soil entomopathogenic nematodes, Steinernema carpocapsae, and is pathetic to a wide range of agriculture pests. Several factors associated to the fermentation of X. nematophila HB310 were measured and optimized in this study. Glucose, beef extract - peptone and KzHPO4 were respectively chosen as the optimum carbon, nitrogen sources and inorganic salt using single factor method. According to the orthogonal experiment, the optimal fermentation medium was identified as： 2% glucose, 1% beef extract- peptone, 0.3% beef extract and 1% K2HPO4. The optimized shake -flashing culture conditions were inoculum size of 6% , pH 7.5, 200 rpm rotary speed, 28℃ and 48h fermentation time. Under this condition, the X. nematophila HB310 showed the fastest growth rate and the highest insecticidal activity.

关 键 词：嗜线虫致病杆菌 发酵优化 正交试验 小菜蛾 

分 类 号：Q965[生物学—昆虫学] S476[农业科学—农业昆虫与害虫防治]