绞股蓝皂苷对AGEs诱导人肾小球系膜细胞增殖及其TGF-β_1、FN mRNA表达的影响  

Effect of gypenosides on the proliferation of HMCs and expressions of TGF-β_1 and FN mRNA induced by AGEs

作　　者：周康[1] 唐灵[1] 肖福英[2] 王艳[1] 

机构地区：[1]桂林医学院附属医院,广西桂林541001 [2]桂林医学院

出　　处：《山东医药》2014年第47期9-12,共4页Shandong Medical Journal

基　　金：广西自然科学基金资助项目(2013GXNSFAA019197);桂林市科学研究与技术开发计划项目(20120121-1-1)

摘　　要：目的观察绞股蓝皂苷(GP)对晚期糖基化终末产物(AGEs)诱导人肾小球系膜细胞(HMCs)增殖及其转化生长因子β1(TGF-β1)、纤维连接蛋白(FN)mRNA的影响。方法将对数生长期HMCs细胞分为A、B、C、D组。A、B、C组均采用200 mg/L AGEs诱导HMCs,B组同时给予不同剂量GP干预,C组同时给予0.1 mmol/L氨基胍盐酸盐;D组不给予任何诱导和干预。采用MTT法观察HMCs增殖情况,RT-PCR法检测HMCs中TGF-β1、FN mRNA。结果与D、A组比较,B组HMCs增殖抑制率升高,且呈剂量、时间依赖性(P均<0.01);与C组比较,B组以25、50、75、100 mg/L GP干预后HMCs增殖抑制率降低,以150、200 mg/L GP干预后HMCs增殖抑制率升高,且呈剂量、时间依赖性(P均<0.01)。与D组比较,B组TGF-β1、FN mRNA表达升高,且呈剂量依赖性(P均<0.01);与A组比较,B组TGF-β1、FN mRNA表达降低,且呈剂量依赖性(P均<0.01)。结论 GP可抑制AGEs诱导HMCs过度增殖,其机制可能与下调TGF-β1、FN mRNA过度表达有关。Objective To investigate the effect of gypenosides （ GP） on the proliferation of human mesangial cells （ HMCs） and the expression of TGF-β1 and FN mRNA induced by AGEs .Methods Logarithmic growth phase HMCs were divided into A, B, C, D groups, which were induced by AGEs （200 mg/L） in A, B, C groups, group B was inter-vened by different doses of GP at the same time , while group C was given 0.1 mmol/L aminoguanidine hydrochloride , group D was not given any induction and intervention .The inhibition of HMCs proliferation was determined by MTT assay . The expressions of TGF-β1 and FN mRNA in HMCs in all groups were detected by semi-quantitative RT-PCR.Result Compared with group D and group A , the inhibition of cell proliferation in Group B increased in a dose-and time-dependent manner （all P〈0.01）.Compared with group C,the inhibition of cell proliferation in group B decreased intervened by 25, 50, 75, 100 mg/L GP, while increased by 150, 200 mg/L GP, and in a dose and time dependent manner （all P〈0.01）. Compared with group D , group B increased in a dose-dependent manner （ all P〈0.01）;Ccompared with group A , the ex-pression of TGF-β1,FN mRNA in group B decreased in a dose-dependent manner（all P〈0.01）.Conclusion GP can in-hibit the over proliferation of HMCs induced by AGEs , the mechanism may be related to down-regulate the over-expressions of TGF-β1 and FN mRNA.

关 键 词：绞股蓝皂苷 糖尿病肾病 晚期糖基化终末产物 肾小球系膜细胞 

分 类 号：R587.2[医药卫生—内分泌]