女性不孕不育患者DNT细胞与T细胞亚群变化及意义  

Clinical significance of double- negative regulatory T cells and T cell subsets in female infertility

作　　者：王海芳[1] 吴晓娟[1] 何宗忠[2] 王红霞[1] 李海侠[1] 裘宇容[1] 

机构地区：[1]南方医科大学南方医院检验科,广州510515 [2]广州军区广州总医院附属一五七医院检验科,广州510515

出　　处：《广东医学》2014年第23期3654-3657,共4页Guangdong Medical Journal

基　　金：广东省教育厅科研项目(编号:2012KJCX0029)

摘　　要：目的探讨女性不孕不育患者外周血CD3+CD4-CD8-双阴性调节性T细胞(DNT细胞)与T细胞亚群变化的临床意义。方法选择106例女性不孕不育患者,其中不孕症36例,复发性流产(RSA)70例,≤30岁56例,>30岁50例。根据狼疮抗凝初筛实验结果、狼疮抗凝确认实验结果、抗精子抗体实验结果分组统计。另选38例健康体检女性作为对照。采用流式细胞术检测女性不孕不育患者和健康体检女性外周血DNT细胞与T细胞亚群。结果与健康体检女性相比,不孕不育患者CD3+、CD3+CD4+CD8-、CD3+CD4-CD8+、NK细胞的差异均无统计学意义(P>0.05),DNT细胞的差异有统计学意义(P<0.001),其中RSA患者、不孕症患者的DNT细胞均显著高于健康体检女性,差异有统计学意义(P<0.001),而RSA患者与不孕症患者的DNT细胞差异无统计学意义(P=0.090)。≤30岁患者CD3+、CD3+CD4+CD8-、CD3+CD4-CD8+、DNT、NK细胞与>30岁患者差异均无统计学意义(P>0.05)。狼疮抗凝物(LA)初筛阴性患者的DNT细胞高于LA初筛阳性患者,差异有统计学意义(P=0.047),而CD3+、CD3+CD4+CD8-、CD3+CD4-CD8+、NK细胞的差异均无统计学意义(P>0.05)。LA确认阴性患者的DNT细胞低于LA确认阳性患者,差异有统计学意义(P=0.017),而CD3+CD4-CD8+LA确认阴性患者高于确认阳性患者,差异也有统计学意义(P=0.007),而CD3+、C3+CD4-CD8+、NK细胞的差异均无统计学意义(P>0.05)。CD3+、CD3+CD4+CD8-、CD3+CD4-CD8+、DNT与NK细胞在抗精子抗体(As Ab)阴性患者与AsAb阳性患者的差异无统计学意义(P>0.05)。结论不孕不育患者DNT细胞升高与T细胞亚群的变化提示机体免疫功能异常,在不孕不育的发病机制中可能起一定作用。Objective To investigate the clinical significance of CD3 - CD4 - CD8 double negative regulatory T cells （DNT） and T cell subsets in female infertility. Methods A total of 106 female infertility patients, among whom 36 was infertility and 70 was recurrent spontaneous abortion （RSA）. And 56 cases were less than 30 years old. Grouping statistics was performed according to the result of lupus anticoagulant screening, lupus anticoagulant confirm and anti sperm antibody. Thirty eight healthy women were selected as controls. DNT and T cell subsets were detected by flow cytometry. Results Compared with normal control group, there was no significant difference of CD3 - , CD3 ＋ CIM ＋ CD8 , CD3 ＋ CD4 - CD8 ＋ or NK ceils in female infertility patients （ P 〉 0.05 ） , while the percentage of DNT ceils were significantly increased in infertility patients compared with normal controls （ P 〈 0. 001 ）. In infertility patients, the percentage of DNT cells in RSA patients and sterility patients were significantly higher than that in normal controls （P 〈 0. 001 ）, there was no significant difference between RSA and sterility patients （ P = 0. 090）. There was no significant difference in the percentage of CD3 ＋ , CD3 ＋ CIM ＋ CD8 ＋ , CD3 ＋ CIM - CD8 ＋ , DNT or NK cells between female infertility patients elder and younger than 30 years （ P 〉 0. 05 ）. The percentage of the DNT cells was significant higher in screening LA negative patients than that in positive patients （ P = 0. 047 ） , while there was no significant difference in the percentage of the CD3 ＋ , CD3 ＋ CIM ＋ CD8 , or NK cells （ P 〉 0. 05 ）. The percentage of the DNT cells was significant higher while the percentage of CD3 ＋ CD4 - CD8 ＋ was significantly lower in lupus anticoagulant confirm negative patients than positive patients （P = 0. 017 and 0. 007 ） ; otherwise there was no significant difference in the percentage of the CD3 ＋ , CD3 ＋ CD4 - CD8 ＋ or NK cells （ P 〉 0. 05 ）. Ther

关 键 词：CD3+CD4-CD8-双阴性调节性T细胞 不孕不育 复发性流产 

分 类 号：R722.1[医药卫生—儿科]