鹿龙再生汤对再障小鼠外周血单个核细胞及脾teff细胞T-bet和GATA-3基因表达的影响  

The effects of Lulongzaisheng soup on T- bet and GATA- 3 gene expression in peripheral blood mononuclear cells and spleen teff cells from mouse model of aplastic anemia

作　　者：钱宇[1,2] 姜胜华[1] 林赠华[1] 刘红[1] 王旭丽[1] 刘海燕[1] 孙峰[1] 

机构地区：[1]南通大学附属医院血液内科,江苏南通226001 [2]河北省秦皇岛市第一人民医院儿一科,066000

出　　处：《广东医学》2014年第24期3783-3787,共5页Guangdong Medical Journal

基　　金：国家自然科学基金资助项目(编号:81070400);江苏省中医药局科技项目(编号:LZ11096);江苏省南通市科技项目临床医学科技专项(编号:HS2013067);江苏省南通市科技项目(编号:S2010023)

摘　　要：目的探讨鹿龙再生汤对再生障碍性贫血(简称再障)模型小鼠外周血单个核细胞以及脾CD4+CD25-细胞(teff细胞)中T-bet、GATA-3基因表达的影响。方法通过IFN-γ腹腔注射加白消安灌胃的方法建造40只再障小鼠模型,分别予同体积的生理盐水、一般浓度、5倍浓度和10倍浓度的鹿龙再生汤进行灌胃治疗,分别归为再障组、再障小剂量组、再障中剂量组和再障大剂量组(n=10);另取40只正常小鼠按上述方法进行分组处理,分别归为正常组、正常小剂量组、正常中剂量组和正常大剂量组(n=10)。通过骨髓活检病理学检查观察用药后各组小鼠的骨髓造血变化,免疫磁珠分选各组小鼠脾teff细胞,利用RT-PCR技术检测各组小鼠外周血单个核细胞以及脾teff细胞T-bet、GATA-3基因mRNA的表达情况。结果 (1)与再障组相比,再障中剂量组和再障大剂量组小鼠的骨髓造血有所恢复,尤其是再障大剂量组。(2)再障大剂量组小鼠的外周血单个核细胞以及脾teff细胞T-bet基因mRNA的表达、T-bet/GATA-3的比值较再障组均明显下降(P<0.05),而GATA-3基因mRNA的表达明显上调(P<0.05)。结论大剂量鹿龙再生汤可改善再障小鼠骨髓造血功能,可能是通过调节再障小鼠外周血单个核细胞及脾teff细胞的T-bet、GATA-3基因的表达来实现。Objective To study the effects of Lulongzaisheng soup on T - bet and GATA - 3 gene expression in peripheral blood mononuelear cells and spleen teff cells from mouse model of aplastic anemia （AA）. Methods Mice were randomly selected to set up the AA model using intraperitoneal injection with IFN -a and intragastric administration with busulphan. Both the normal mice and the AA mice were divided into low - dose, middle - dose and high - dose groups according to the different dose soups used, which were equaling to the same concentration in human begings, 5 times of the concentration and 10 times of the concentration, respectively （ n = 10 ）. Bone marrow hematopoiesis changes were observed by marrow biopsy pathology test. T - bet and GATA - 3 gene expression in peripheral blood mononuclear cells and spleen teff cells pureed by immunomagnetic beads were detected by using RT - PCR. Results In AA middle - dose and high - dose groups, moderate improvements of marrow hematopoiesis in AA mice were found, compared with AA group, especially in the AA high dose group. Compared with AA group, the T - bet/GATA - 3 and the mRNA expression of T - bet gene in both peripheral blood mononuclear cells and spleen teff cells were significantly reduced in AA high - dose group, while the mRNA expression of GATA - 3 was significantly increased （ P 〈 0.05 ）. Conclusion The high dose Lulongzaisheng soup may improve marrow hematopoiesis in AA mice by regulating T - bet and GATA - 3 gene expres- sion in oerioheral blood mononuclear cells and soleen teff cells.

关 键 词：鹿龙再生汤 再生障碍性贫血 动物模型 teff细胞 T-BET GATA-3 

分 类 号：R512.62[医药卫生—内科学]