大叶南五味子多糖对鸡免疫细胞增殖的影响  

Effects of Polysaccharides from Kadsura marmorata on Proliferation of Immune Cells in Chickens

作　　者：王宏军[1] 周铁忠[1] 侯艳杰 刘英姿[1] 杨松[1] 蒋红[1] 

机构地区：[1]辽宁医学院,辽宁锦州121001 [2]辽宁凯为生物技术有限公司,辽宁锦州121000

出　　处：《中国家禽》2014年第24期18-22,共5页China Poultry

基　　金：国家自然科学基金项目(31201951);辽宁省高等学校优秀人才支持计划(LJQ2013087);国家科技型中小企业创新基金项目(13C26212100952)

摘　　要：试验将5种浓度大叶南五味子多糖（KPS）5个组分单独加入鸡的T、B淋巴细胞及腹腔巨噬细胞（PMφ）培养体系中，培养44h时用MTT法测定免疫细胞增殖（OD599值）的变化。结果显示，在150～250μg／mL时，KPSⅡ、KPSⅢ组T淋巴细胞增殖OD599值显著高于空白对照组、ConA对照组（P〈0．05）；在50～100μg／mL时，KPSⅡ、KPSⅢ组B淋巴细胞增殖OD599值显著高于空白对照组、PHA对照组（P〈0．05）；在50～150μg／mL时，KPSⅡ、KPSⅢ、KPSⅣ-6组腹腔巨噬细胞增殖OD599值显著高于空白对照组、LPS对照组（P〈0．05）；KPS各组分在50-250μg／mL时均能促进鸡T、B淋巴细胞及PMφ细胞增殖，且在同等剂量下KPS促进B淋巴细胞增殖OD599值高于其他细胞。结果表明，KPS在合适的剂量能单独刺激鸡的T、B淋巴细胞及PMφ细胞增殖，从而增强鸡的细胞免疫能力。In this study,five kinds of Kadsura marmorata polysaccharides （KPS）at five concentrations were added to the culture system T lymphocyte,B lymphocyte and peritoneal macrophages （PMφ）of chickens,cuhured for 44 h with MTT method for the determination of cell proliferation （OD599） changes. The results showed that OD599 values of 150 to 250 μg/mL of KPS Ⅱ and KPS Ⅲ groups were significantly higher than those of cell control group and ConA control group （P〈0.05）;OD599 values of 50 to 100 txg/mL of KPS Ⅱ and KPSm groups were significantly higher than those of cell control group and PHA group （P〈0.05）;OD599 values of 50 to 150 p,g/mL of KPS Ⅱ ,KPSm and KPSIV-6 group were significantly higher than those of cell control group and LPS group （P〈0.05）;At 50 to 250 txg/mL of five kinds of KPS groups, OD599 values of KPS groups were higher than those of cell control group. OD599 values of B lymphocyte groups was higher than T lymphocyte and peritoneal macrophages groups under the same doses. The results indicated that KPS at suitable doses could stimulate T,B lymphocyte and peritoneal macrophages proliferation of chicken,thereby enhancing cellular immunity of chicken in vitro.

关 键 词：大叶南五味子多糖 鸡脾脏淋巴细胞 鸡腹腔巨噬细胞 增殖 

分 类 号：S853.74[农业科学—临床兽医学]