灯盏花素在不同时间点对HepG2肝细胞TG含量的影响及机制探讨  

Initial investigation on effect of breviscapine on triglyceride in HepG2 cells in different time points and its mechanism

作　　者：阳琰[1] 高琳[1] 邓华聪[2] 晏永慧[1] 李显文[1] 王小英[1] 廖鑫[1] 张晗[1] 陈其荣[1] 王茜[1] 

机构地区：[1]遵义医学院附属医院内分泌科,563003 [2]重庆医科大学附属第一医院,400016

出　　处：《重庆医学》2014年第36期4852-4853,4856,共3页Chongqing medicine

基　　金：国家自然科学基金资助项目(81460168);贵州省科学技术基金资助项目(黔科合J字LKZ[2013]19号);遵义医学院博士启动基金(F-574);贵州省优秀科技教育人才省长专项资金项目(黔省专合字[2008]110号);贵州省科技合作项目(黔科合区域合[2010]7004号)

摘　　要：目的通过观察灯盏花素在不同时间点对HepG2肝细胞内过氧化物酶体增殖物激活受体-α(PPAR-α)、载脂蛋白A5(apoA5)表达和三酰甘油(TG)含量的影响,旨在初步探讨灯盏花素与PPAR-α、apoA5、TG之间的时间依赖关系,为进一步探索灯盏花素调节TG代谢的具体机制奠定一定的基础。方法在前期实验基础上选取100mmol/L灯盏花素,处理HepG2细胞不同时间点:(1)0h(空白对照)组;(2)6h组;(3)12h组;(4)24h组;(5)36h组;(6)48h组;检测各组细胞内PPAR-α、apoA5基因、蛋白表达水平和TG含量。结果灯盏花素可升高HepG2细胞内PPAR-α、apoA5表达,降低TG含量(P<0.05),并呈时间依赖性。结论灯盏花素能降低肝细胞内TG含量,其机制可能通过使PPAR-α表达水平增加,从而增加apoA5表达水平而实现的。Objective To initially investigate the time - dependent relation between breviscapine with peroxisome proliferator‐ac‐tivated receptor‐alpha（PPAR‐α） ,apolipoprotein A5 （apoA5） and triglyceride（TG） in HepG2 cells in different time points by ob‐serving the effect of breviscapine on the expression and contents of PPAR‐α ,apoA5 and TG in order to lay a certain foundation for further exploring the concrete mechanism for its regulating TG metabolism .Methods On the basis of earlier stage experiment ,100 mmol/L breviscapine was selected to treat the HepG2 cells at different time points （0 ,6 ,12 ,24 ,36 ,48 h） .The levels of PPAR‐αand apoA5 gene and protein ,and the TG content in HepG2 cells were detected .Results Breviscapine could increase the levels of PPAR‐α and apoA5 gene and protein and decrease the TG content in HepG2 cells （P〈 0 .05） ,moreover which showed the time -dependence .Conclusion Breviscapine may decrease the TG level in HepG2 cells ,its mechanism may be realized by increasing the expression of PPAR‐α ,thus increacing the expression of apoA5 in HepG2 cell .

关 键 词：灯盏花素 过氧化物酶体增殖物激活受体-Α 载脂蛋白 A5 三酰甘油 

分 类 号：R285[医药卫生—中药学]