湿地松、加勒比松种质资源遗传多样性分析  

Analysis of Genetic Diversity of Pinus elliottii and P. caribaea Germplasm Resources

作　　者：李义良[1] 赵奋成[1] 李宪政[1] 吴惠姗[1] 李福明 钟岁英 张应中[1] 蔡坚[1] 郭文冰[1] 

机构地区：[1]广东省林业科学研究院,广东广州510520 [2]广东省台山红岭种子园,广东台山529200

出　　处：《广东林业科技》2014年第6期9-14,共6页Forestry Science and Technology of Guangdong Province

基　　金：广东省林业科技创新专项项目(2012KJCX009-02);国家林业局林业公益性行业科研专项(20110414)

摘　　要：应用SSR与SRAP分子标记技术分析22份湿地松和28份加勒比松种质资源（其中包括15份洪都拉斯加勒比松样品、9份古巴加勒比松样品、4份巴哈马加勒比松样品）的遗传多样性，结果表明，在SSR分析中，14对SSR引物产生的多态性比率为89％，平均每对引物获得多态性条带4．3条；SRAP分析中，12对SSR引物产生的多态性比率为72％，平均每对引物获得多态性条带2．8条；SSR和SRAP标记综合分析表明，参试材料遗传距离变化范围为0．075-0．633，种质资源间存在丰富的遗传多样性。Genetic diversity was evaluated by sequence-related amplified polymorphism （SRAP）and sim-ple sequence repeat （SSR）markers among 22 germplasm materials of Pinus elliottii,and 28 germplasm materials of P.caribaea including 15 germplasm materials of P.caribaea var. hondurensis, 9 germplasm materials of P.caribaea var.caribaea,and 4 germplasm materials of P.caribaea var.bahmaensis.14 SSR primers produced a polymorphism ratio of 89% with an average of 4.3 fragments per primer,whereas 12 SRAP primer combinations produced a polymorphism ratio of 72% with an average of 2.8 fragments per primer combinations.Data of SRAP and SSR makers,analysed by unweighted pair-group method arithmetic averages,indicated that the accessions had a genetic distance from 0.075 to 0.633,which could fully showed that they have rich genetic diversity.

关 键 词：湿地松 加勒比松 遗传多样性 SSR SRAP 

分 类 号：S791.24[农业科学—林木遗传育种]