西南桦染色体加倍的影响因子初步研究  

Preliminary Study of Predominant Factors on Chromosome Doubling of Betula alnoides

作　　者：陈荣[1,2] 朱昌叁[2] 庞正轰[2] 

机构地区：[1]亳州师范高等专科学校,安徽亳州236800 [2]广西生态工程职业技术学院,广西柳州545004

出　　处：《亚热带植物科学》2014年第4期286-288,共3页Subtropical Plant Science

基　　金：2012年度广西高等学校一般资助科研项目(No.201203YB201)

摘　　要：以西南桦种子无菌萌发小苗的顶芽为外植体,探讨秋水仙素的浓度、处理时间及预培养时间对西南桦染色体加倍的影响,初步建立西南桦染色体加倍的方法。结果表明,使西南桦染色体加倍的秋水仙素浓度以120 mg·L-1为宜;在秋水仙素加倍处理前,预培养10 d后再加倍15 d效果较好,此时得到的西南桦嵌合体较多。In this paper, a method for chromosome doubling of Betula alnoides was developed. Terminal buds from aseptic germination of B. alnoides were used as explants, effect factors on chromosome doubling of B. alnoides were discussed, such as concentration of colchicine, co-culture time of colchicine, preculture time. The results showed that the concentration of colchicine was better to be 120 mg·L^-1 for chromosome doubling of B. alnoides, and maximum chimaera number would be obtained in the regermination plantlet of B. alnoides, after preculture without colchicine for 10 d and then co-culture with colchicine for 15d.

关 键 词：西南桦 秋水仙素 浓度 染色体 

分 类 号：Q343.244[生物学—遗传学]