金钗石斛♀×细茎石斛♂杂交F_1代的离体快繁与试管开花(简报)  

Rapid Propagation of the Hybrid Seeds of Den. nobile♀× Den. moniliforme♂ and Flowering in vitro

作　　者：卓孝康 陈燕琼[1,2] 李淑娴[1,2] 漆子钰 彭东辉[1,2] 兰思仁[1,2] 

机构地区：[1]福建农林大学园林学院,福建福州350002 [2]福建农林大学海峡兰花保育研究中心,福建福州350002

出　　处：《亚热带植物科学》2014年第4期327-328,共2页Subtropical Plant Science

基　　金：林业公益性行业科研专项(201204604);福建省种业创新与产业化工程项目(2014S1477-7)资助

摘　　要：以金钗石斛♀×细茎石斛♂杂交F1代种子为材料,探索两种石斛杂交后代的快繁技术和诱导试管开花。结果表明,适于种子萌发的培养基为1/2MS＋6-BA 1.0 mg·L^-1＋NAA 2.0 mg·L^-1＋IBA 2.0 mg·L^-1＋香蕉100 g·L^-1;继代增殖培养基为1/2MS＋6-BA 0.2 mg·L^-1＋NAA 1.0 mg·L^-1＋香蕉100 g·L^-1,增殖系数达4-5倍;不定芽诱导培养基为1/2MS＋6-BA 2.0 mg·L^-1＋NAA 0.2 mg·L^-1＋蛋白胨2 g·L^-1,诱导率达93.3%,诱导系数达3.7;生根培养基为TH＋6-BA 0.5 mg·L^-1＋NAA 1.0 mg·L^-1,生根率可达91.7%,根数3-5条;花芽诱导培养基为6-BA2.0 mg·L^-1＋NAA 0.5 mg·L^-1＋IBA 0.5 mg·L^-1,花芽诱导率达8%。The F1hybrid seeds ofDen. nobile♀ ×Den. moniliforme♂ were taken as materials to explorein vitro rapid propagation techniques and flowering. The results showed that the optimum media for seedlings germination was 1/2MS ＋ 6-BA 1.0 mg·L^-1 ＋ NAA 2.0 mg·L^-1＋ IBA 2.0 mg·L^-1＋ banana mud 100 g·L^-1. The optimum medium for proliferation of subculture was 1/2MS ＋ 6-BA 0.2 mg·L^-1＋ NAA 1.0 mg·L^-1 ＋ banana mud 100 g·L^-1 with the proliferation coefficient of 4—5 times. The medium for regeneration of adventitious buds was 1/2MS ＋ 6-BA 2.0 mg·L^-1＋ NAA 0.2 mg·L^-1＋ peptone 2 g·L^-1 with 93.3% induction rate and 3.7 times induction coefficient. The rooting medium was TH ＋ 6-BA 0.5 mg·L^-1＋ NAA 1.0 mg·L^-1, the rooting rate was up to 91.7% with 3—5 roots. The medium for the floral bud induction was6-BA 2.0 mg·L^-1＋ NAA 0.5 mg·L^-1 ＋ IBA 0.5 mg·L^-1 with the rate of 8%.

关 键 词：石斛 杂交 离体快繁 试管开花 

分 类 号：Q943.1[生物学—植物学] Q321.2