跨代染镉对子代大鼠大脑引起的脂质过氧化  

Effects of continuous administration of cadmium from the parental generation to first filial generation on lipid peroxidation damage in rats

作　　者：张燕[1] 王克跃[1] 

机构地区：[1]遵义医学院公共卫生学院毒理学教研室,贵州遵义563099

出　　处：《遵义医学院学报》2014年第6期609-611,共3页Journal of Zunyi Medical University

基　　金：贵州省科学技术基金项目(NO:黔科合J字[2013]2317)

摘　　要：目的探讨跨代低剂量镉暴露对子代大鼠引起的氧化损伤。方法将36只8周龄SD大鼠随机分为4组:饲料中不添加镉组(1组);饲料中按1mg/kg加入镉组(2组);饲料中按5mg/kg加入镉组(3组)、饲料中按25mg/kg加入镉组(4组)。雌雄分开饲养,1月后按照雌雄2∶1的比例合笼,产下的幼鼠同亲代同组大鼠镉暴露方式和剂量继续接触镉,待幼鼠成长至8周,测定子代大鼠脑组织中丙二醛(MDA)的含量,测定亲代和子代大鼠肝、肾、脑中镉含量。结果与1组相比,2、3、4组各组织中镉含量均增高,脑组织中MDA的含量升高,但脑中锌含量降低(P<0.01或P<0.05)。脑组织中MDA含量与脑中镉含量(r=0.69,P<0.05)、肝脏中镉含量(r=0.58,P<0.05)和肾脏中镉含量(r=0.78,P<0.05)呈正相关,与脑中锌含量呈负相关(r=-0.11,P<0.05)。结论低剂量跨代镉暴露能引起子代大鼠大脑脂质过氧化损伤。Objective To study the effects of continuous administration of cadmium（ Cd） from the parental generation to first filial generation on lipid peroxidation damage in rats. Methods Thirty- six eight- week- old SD rats were randomly divided into four groups： no cadmium in feed group（ group 1）,cadmium（ 1 mg / kg） in feed group（ group 2）,cadmium（ 5 mg / kg） in feed group（ group 3） and cadmium（ 25 mg / kg） in feed group（ group 4）. Male and female rats were fed separately. One month later,two male and one female rats in the same group were placed in one cage to obtain pregnant rats. After ablactation,the offsprings were given the same feed as their parents until 8 weeks. The contents of malondialdehyde（ MDA） and Zinc（ Zn） in the brain and Cd in the kidney,liver and brain of the parents and offsprings were measured. Results Compared with group 1,the content of Cd was increased and Zn level was decreased in the brain and the content of MDA in the brain was increased in 2,3 and 4 groups. MDA content in the brain was significantly positively correlated to brain Cd（ r =0. 69,P〈0. 05）,renal Cd（ r = 0. 78,P〈0. 05） and liver Cd（ r = 0. 58,P〈0. 05） and significantly negatively correlated to brain Zn（ r =- 0. 11,P〈0. 05）. Conclusion Continuous administration of Cd from the parental generation to first filial generation could result in rat brain lipid peroxidation damage.

关 键 词：镉 锌 丙二醛 

分 类 号：R69[医药卫生—泌尿科学]