重楼皂苷对微囊藻毒素致小鼠肝损伤保护作用的组织学研究  

Histological Study of Protective Effect of the Saponins in Rhizoma Paridis Yunnanensis on Mice Liver Injury Induced by Microcystin

作　　者：杨黎江[1] 路斌[1] 沈放[1] 杨晶[1] 马银海[2] 

机构地区：[1]昆明学院生命科学与技术系,云南昆明650214 [2]昆明学院化学科学与技术系,云南昆明650214

出　　处：《昆明学院学报》2014年第6期36-38,41,共4页Journal of Kunming University

基　　金：昆明学院科学研究基金资助项目(XJL12023)

摘　　要：试验用重楼薯蓣皂苷(SY)和偏诺皂苷(PN)对微囊藻毒素浸染的小鼠进行连续灌胃,并通过对小鼠肝脏进行石蜡包埋、制片和HE染色,观察小鼠肝组织的显微结构,目的在于研究重楼皂苷类化合物对微囊藻毒素(MC)所致肝损伤的保护作用.结果显示,微囊藻毒素具有肝毒性,致肝小叶结构严重破坏,而重楼薯蓣皂苷和偏诺皂苷对微囊藻毒素所致的肝损伤均有保护作用.Mouse induced by microcystin was chronically intragastric lavement by dioscins and pennogenyl saponins.The liver of the mouse was embeded in paraffin and made slice for HE staining to observe the liver tissue micro structure of the mouse and the purpose is to study whether the saponins in Rhizoma Paridis Yunnanensis prevent liver from damage induced by microcystin.The results showed that microcystin is hepatotoxic and induce serious collapse of lobular architecture;but both dioscins and pennogenyl saponins can protect liver from damage induced by microcystin.

关 键 词：微囊藻毒素 小鼠肝脏 薯蓣皂苷 偏诺皂苷 HE染色 

分 类 号：R994.6[医药卫生—毒理学]