HPLC法测定千柏鼻炎片中的金丝桃苷  

Determination of Hyperoside in the Qianbai Biyan Pian by HPLC

作　　者：苏健 

机构地区：[1]昆明市食品药品检验所,云南昆明650032

出　　处：《昆明学院学报》2014年第6期80-81,91,共3页Journal of Kunming University

摘　　要：采用HPLC外标法测定10批千柏鼻炎片中的金丝桃苷,色谱条件为,十八烷基硅烷键合硅胶为填充剂,V(乙腈)∶V(0.2%醋酸溶液)=16∶84为流动相,柱温为30℃,流速为1 m L/min,检测波长为360 nm.在此条件下色谱柱的理论塔板数(n)大于10 000,目标成分色谱峰的分离度(R)大于1.5,拖尾因子(T)大于0.95、小于1.05,色谱条件均能达到《中华人民共和国药典》2010年版中高效液相色谱法的定量检测要求,满足该复方制剂中单一有效成分的定量测定.测定结果表明,10批千柏鼻炎片中的金丝桃苷平均为每片0.848 6 mg.平均回收率为96.90%,RSD为0.41%(n=9).Determining Hyperoside from ten batches of qianbaibiyan tablets with HPLC external standard method,the chromatography condition was eighteen alkyl silane bonded silica as filler,V（acetonitrile）∶V（0.2% acetic acid solution）=16∶84 as the mobile phase;the column temperature was 30℃;the flow rate was 1 mL/min;the detection wavelength was 360 nm.Theoretical plate column under this condition number（n）is greater than 10 000;the degree of separation of the target component chromatographic peak（R）greater than 1.5;the tailing factor（T）greater than 0.95 and less than 1.05;require quantitative detection of chromatographic conditions were able to achieve the“Pharmacopoeia of the people′s Republic of China”in 2010 edition of high performance liquid chromatography a the quantita-tive determination and meet the single active ingredient in the compound preparation.The determination results show that Hyperoside in each tablet of the ten batches in qianbaibiyan tablets averaged 0.848 6 mg.The average recovery was 96.90%,RSD was 0.41%（n=9）.

关 键 词：千里光 千柏鼻炎片 金丝桃苷 HPLC法 

分 类 号：R284[医药卫生—中药学] O657.72[医药卫生—中医学]