麝香乌龙丸对糖尿病兔骨折愈合的影响及其机制探讨  

Effects and mechanisms of Mask Dark Dragon Pelle on diabetes mellitus rabbits fracture healing

作　　者：梁珊[1] 何畔[1] 王愉思[1] 

机构地区：[1]湖南省人民医院,长沙410005

出　　处：《山东医药》2014年第48期13-15,I0017,共4页Shandong Medical Journal

基　　金：湖南省医药卫生科研计划课题项目(B2011-079)

摘　　要：目的：观察麝香乌龙丸对糖尿病（ DM）兔骨折愈合的影响，并探讨其机制。方法将36只大白兔随机分为A、B、C组各12只。 B、C组建立DM模型，3组均造成右胫腓骨骨折。 C组将麝香乌龙丸以1．0 g／kg剂量溶于5 mL生理盐水中灌胃，A、B组给予等量生理盐水灌胃。3组分别于给药后第1、2、4周各取4只大白兔，麻醉后采用Norland．XR 36双能X线骨密度测量仪检测骨折端骨密度，取骨痂行HE染色观察形态学变化，采用Western blot法检测骨痂神经生长因子（ NGF）相对表达。结果 B组局部骨密度在骨折愈合过程中出现下降，给药1～2周时略微下降，第4周下降明显，其下降趋势较A、C组更加明显。 B组各时点骨密度均低于A、C组，P均＜0．05。3组用药后第1周的骨痂形态学均无明显变化；用药第2、4周与B组比较，A组可见大量较成熟软骨存在，形成软骨岛，骨小梁生成并发育成熟；C组未见软骨岛存在，但软骨性骨痂加速向骨性骨痂转化。 B、C组用药第2、4周的骨痂NGF相对表达均较第1周升高，B组各时点骨痂NGF相对表达均低于A、C组，P均＜0．05；A、C组各时点骨痂NGF相对表达比较，P均＞0．05。结论麝香乌龙丸可以加速DM兔骨折愈合，可能与上调骨痂NGF的表达有关。Objective To observe the effects and mechanisms of Mask Dark Dragon Pelle on diabetes mellitus （ DM） rabbits fracture healing.Methods A totle of 36 rabbits were divided into A, B, C groups, 12 rabbits in each group.B and C groups were DM models , A group was normal control .Then fracture of the right tibia and fibula were builted in three groups.The rabbits in C group were received intragastric administration of 1.0 g/kg Mask Dark Dragon Pelle ＋5 mL nor-mal saline, the equal volume of normal saline were given in A and B groups .At 1, 2, 4 weeks after treatment, four rabbits in each group were taken to study .The bone mineral density （ BMD ） was detected by dual-energy X-ray absorptiometry （ DEXA）;and the callus were collected for HE staining to detect pathological changes ;the level of NGF in callus was test-ed by Western blot .Results BMD in B group showed an downward trend during the process of fracture healing , the BMD in B group decreased than A and C groups at 1, 2, 4 weeks （P均〈0.05）.Among three groups, group C displayed the best callus formation in pathological changes , and fracture healing was significantly better than that in the other two groups . At the same time, the expression of NGF was significantly higher in group C than that in group B （ P〈0.05）.Conclu-sions The expression of NGF decreased in callus of DM rabbits .Mask Dark Dragon Pelle accelerates the DM fracture healing, its mechanism maybe related to NGF expression up-regulation.

关 键 词：骨折 糖尿病 麝香乌龙丸 神经生长因子 兔 

分 类 号：R285.5[医药卫生—中药学]