pyrG基因对西瓜噬酸菌致病性和组氨酸利用的影响  

Effect of pyrG gene on pathogenicity and utilization of histidine in Acidovorax citrulli

作　　者：赵玉强[1] 田艳丽[2] 高杜娟[2] 罗金燕[1] 邱晓静[2] 胡白石[2] 

机构地区：[1]上海市农业技术推广服务中心,上海201103 [2]南京农业大学植物保护学院/农作物生物灾害综合治理教育部重点实验室,江苏南京210095

出　　处：《江苏农业学报》2014年第6期1309-1315,共7页Jiangsu Journal of Agricultural Sciences

基　　金：上海市农业技术推广服务中心科技发展基金项目[3031344]

摘　　要：为研究pyr G基因与西瓜噬酸菌(Acidovorax citrulli)致病相关性,利用同源重组的方法构建pyr G基因突变体(Δpyr G),对pyr G基因的功能进行分析。结果显示,pyr G基因影响病原菌的致病性、游动性、生物膜形成和定殖能力以及激发烟草过敏反应能力;pyr G基因影响his D基因的表达;此外,pyr G基因突变影响西瓜噬酸菌对组氨酸的利用。To study the relationship between pyr G and Acidovorax citrulli pathogenicity,pyr G gene mutant Δpyr G was constructed by homologous recombination method,and the function of pyr G gene was analyzed.Δpyr G from wild-type strain Acxj L12 in pathogenicity swimming motility,biofilm formation,colonization ability and hypersensitive response to tobacco.qRT-PCR revealed that pyr G affected the transcription of his D gene.The pyr G gene mutation played a negative role in histidine utilization by Acidovorax citrulli.

关 键 词：西瓜噬酸菌 PYR G基因 致病性 组氨酸 

分 类 号：S432.1[农业科学—植物病理学]