转拟南芥P5CS1基因羽衣甘蓝的抗寒性  

Cold tolerance of Arabidopsis thaliana P5CS1 transgenic Brassica oleracea L.

作　　者：李大红[1] 焦江洪[1] 赵丽[2] 李鸿雁[1] 

机构地区：[1]黄淮学院生物工程系,河南驻马店463000 [2]黄淮学院园林绿化中心,河南驻马店463000

出　　处：《江苏农业学报》2014年第6期1442-1447,共6页Jiangsu Journal of Agricultural Sciences

基　　金：河南省科技发展计划项目(112300410042)

摘　　要：为提高羽衣甘蓝的抗寒性,本试验将拟南芥△1-吡咯啉-5-羧酸合成酶(P5CS1)基因经农杆菌介导转入羽衣甘蓝植株中,检测转基因株系在4℃低温胁迫下P5CS1 mRNA表达量、幼苗脯氨酸含量、可溶性糖含量、相对电导率、叶绿素荧光参数(Fv/Fm)和植株存活率。结果显示,在4℃低温胁迫下,转基因植株的P5CS1基因的mRNA过量表达,脯氨酸含量、可溶性糖含量、Fv/Fm和植株存活率均显著高于野生型对照(P<0.05),但丙二醛含量和相对电导率均显著低于野生型对照(P<0.05)。表明拟南芥P5CS1基因在羽衣甘蓝中的表达明显改善了转基因植株的抗寒性。A pyrroline-5-carboxylate synthetase（P5CS1） c DNA from Arabidopsis thaliana was transferred to Brassica oleracea plants via agrobacterium mediated transformation.Proline content,soluble sugar content,relative conductivity,Fv/Fmand plant survival rate of transgenic seedlings and wild type seedlings（control） were detected under the stress of 4℃.mRNA of P5CS1 gene was expressed in transgenic plants.The proline content,soluble sugar content,Fv/Fmand plant survival rate of transgenic plants were significantly higher than those of control（P〈0.05）,however,malondialdehyde content and relative conductivity were much lower（P〈0.05）.Arabidopsis thaliana P5CS1 gene expression significantly improved the cold resistance of transgenic Brassica oleracea.

关 键 词：△1-吡咯啉-5-羧酸合成酶(P5CS1) 羽衣甘蓝 脯氨酸 抗寒性 

分 类 号：S635.9[农业科学—蔬菜学]