黄芪甲苷对肾间质纤维化的拮抗作用  

Antagonistic Effect of Astragalus Saponin I on Renal Interstitial Fibrosis

作　　者：朱祎[1] 唐英[1] 何立群[1,2,3] 

机构地区：[1]上海中医药大学附属曙光医院,上海200021 [2]上海市中医临床重点实验室,上海200021 [3]上海市高校中医内科E-研究院,上海200021

出　　处：《辽宁中医杂志》2014年第12期2700-2702,共3页Liaoning Journal of Traditional Chinese Medicine

基　　金：国家自然科学基金项目(81173219);科技部中医药行业科研专项(201007005);上海市科委创新行动计划项目(11DZ1973100);上海高校创新团队建设项目

摘　　要：目的:探讨黄芪甲苷对大鼠单侧输尿管梗阻模型(UUO)肾间质纤维化的影响。方法:27只雄性SD大鼠随机分为假手术组、模型组、黄芪甲苷组,每组9只。于治疗2周后,心脏采血,检测血肌酐、尿素氮;取梗阻侧肾组织,免疫组织化学染色观察TGF-β、CTGF、FN的阳性表达,并进行半定量分析。结果:与假手术组相比,模型组血肌酐、尿素氮的水平以及TGF-β、CTGF、FN的表达均显著增高。与模型组相比,黄芪甲苷组血肌酐、尿素氮水平降低,TGF-β、CTGF、FN的阳性表达显著下调。结论:黄芪甲苷可能通过降低大鼠血肌酐、尿素氮,抑制TGF-β、CTGF、FN的表达,改善肾功能作用,从而延缓肾间质纤维化病程的进展。Objective ： To explore the effects of Astragalus Saponin I （ ASI ） on renal interstitial fibrosis following unilateral ureteral obstruction （UUO） in rats. Methods：27 male SD rats were randomly divided into 3 groups ,9 in each group：the sham operated group, UUO group and AS[ group. After treatment for additional 14 days ,the rats were killed to get their blood through heart. The levels of Scr and BUN were measured and the semiquantitative assessments of interstitial fibrosis degree and immunohisto- chemical studies localizing Transforming growth factor - β （ TGF - β ） , Connective tissue growth factor （CTGF） and Fibronectin （FN） were carried out. Results：The levels of Scr and BUN and the expressions of TGF - β, CTGF and FN in the model group were significantly higher than those in the sham operated group. Compared with model group, the levels of Scr and BUN and the expressions of TGF-β, CTGF and FN in the ASI group were obviously decreased. Conclusion：ASI alleviates renal interstitial fibrosis possibly via down regulating the levels of Scr and BUN and the expressions of TGF - β, CTGF and FN to improve the renal function . Keywords Astragalus Saponin I;renal interstitial fibrosis;transforming growth factor - β;connective tissue growth factor; fibroneciin

关 键 词：黄芪甲苷 肾间质纤维化 转化生长因子-Β 结缔组织生长因子 纤维粘连蛋白 

分 类 号：R692[医药卫生—泌尿科学]