肺炎克雷伯菌突变株的分离和全转录组分析  

Isolation and transcriptome sequencing of Klebsiella pneumonia mutant strain

作　　者：刘岩[1] 常德[2] 黄佩佳[1] 姜学革[1] 苏龙翔[1] 岑忠 刘长庭[1] 

机构地区：[1]解放军总医院南楼呼吸科,北京100853 [2]武警总医院呼吸科,北京100039 [3]深圳华大基因研究院,广东深圳518083

出　　处：《解放军医学院学报》2014年第12期1234-1236,1256,共4页Academic Journal of Chinese PLA Medical School

基　　金：国家"973"重点基础研究计划(2014CB744400);武器预研重点基金(9140A26040312JB10078);载人航天领域项目(040203);国家自然科学基金(81350020)~~

摘　　要：目的筛选空间环境诱变的肺炎克雷伯菌突变株及并进行全转录组分析。方法利用Biolog生化反应板从搭载于神舟八号飞船的肺炎克雷伯菌中筛选突变株,然后采用高通量测序技术对突变株全转录组测序,原始测序数据经处理后进行基因表达注释和差异表达基因的基因本体功能及京都基因与基因组信号通路注释分析。结果空间搭载的肺炎克雷伯菌突变株在糖类代谢上出现差异,且该菌株有232个基因表达上调,1 879个基因下调,其主要同细菌的膜蛋白、转运相关功能、糖类的运输相关。结论获得一株空间环境诱导的肺炎克雷伯菌突变株且该菌株全转录组测序和注释清晰,为后续的功能研究奠定了基础。Objective To screen the space environment induced Klebsiella pneumonia mutant strain and study its transcriptome. Methods The Klebsiella pneumonia mutant strain was screened from which loaded on Shenzhou- V^I space craft using Biolog GEN IU Micro Plate TM. Then whole transcriptome was sequenced with high-throughput sequencing technology, and gene expression annotation, GO annotation and KEGG signaling pathway annotation were analyzed for differentially expressed genes with processed raw sequencing data. Results Klebsiella pneumoniae mutant showed differences with controls in carbohydrate metabolism. This mutant strain had 232 genes up regulated and 1879 genes down regulated, which were mainly associated with bacterial membrane proteins, transport- related functions and carbohydrate transport. Conclusion One space environment-induced Klebsiella pneumoniae mutan strain is obtained, and the whole transcriptome sequencing and annotation are clear, which lays foundation for subsequent functional studies.

关 键 词：克雷伯菌 肺炎 转录组 测序 空间环境 

分 类 号：R85[医药卫生—航空、航天与航海医学]