EIIIA^+纤维连接蛋白在大鼠皮肤切创的表达  

Expression of EIIIA^+ Fibronectin in Incised Wound of Rat's Skin

作　　者：贺盟[1] 赵海[2] 沈忆文[1] 薛爱民[1] 陈龙[1] 顾云菊[1] 赵子琴[1] 

机构地区：[1]复旦大学基础医学院法医学系,上海200032 [2]上海市公安局水上公安分局,上海200002

出　　处：《法医学杂志》2014年第6期409-412,共4页Journal of Forensic Medicine

摘　　要：目的探讨大鼠皮肤切创纤维连接蛋白EIIIA片段的表达与损伤时间的关系。方法取48只成年SD大鼠并切割大鼠背部皮肤致伤。于伤后即刻、0.5 h、1 h、2 h、3 h、4 h、6 h、8 h共8个时间段分别处死大鼠,取其创伤周围皮肤,利用免疫组织化学方法和Western印迹法检测EIIIA+纤维连接蛋白,观察其表达水平与损伤时间的关系。结果伤后即刻未出现EIIIA+纤维连接蛋白的表达,伤后0.5 h,皮肤基底细胞开始出现阳性表达,随损伤时间的延长,阳性着色持续增强。Western印迹法检测发现,所测得的相对光密度值随损伤时间的延长而逐渐增高。结论 EIIIA片段表达变化可以作为法医学早期损伤时间的推断指标。Objective To explore the relationship between the expression of EIIIA ＋ fibronectin in incised wound of rat’s skin and injury time. Methods The wounding model was established by cutting the dor-salskin of 48 adult SD rats. The rats were sacrificed atthe pre-setinjury time as immediately, 0. 5h, 1h, 2 h, 3 h, 4 h, 6 h, and 8 h. The skin sam ples were taken at the m argin of wound. The expression of the EIIIA ＋ fibronectin was detected by im m unohistochem istry and W estern blotting and the relationship be-tween its expression and injury time was observed. Results The expression of EIIIA ＋ fibronectin was not observed im m ediately. The basal cell of skin began to showpositive expression 0. 5 h after injury. W ith the extension of injury time, positive staining became stronger. The value of relative optical density was gradually increased with prolonged injury time by the W estern blotting analysis. Conclusion The expres-sion of EIIIA ＋ fibronectin could be used for estimation of injury time in the early stage of skin injury.

关 键 词：法医病理学 创伤和损伤 皮肤 纤维连接蛋白 大鼠 

分 类 号：DF795.1[医药卫生—法医学]