液基细胞学技术应用于血样羊水栓塞的诊断  

Diagnosis of Amniotic Fluid Embolism with Blood Samples by Liquid-based Cytology Technique

作　　者：刘宝琴[1] 邓建强[2] 侯安潮 蔡继峰[4] 

机构地区：[1]海南医学院附属医院生殖医学中心,海南海口570102 [2]海南医学院法医鉴定中心,海南海口570102 [3]眉县人民医院,陕西宝鸡721100 [4]中南大学湘雅医学院法医学系,湖南长沙410078

出　　处：《法医学杂志》2014年第6期416-418,共3页Journal of Forensic Medicine

基　　金：国家自然科学基金资助项目(81260465)

摘　　要：目的建立基于液基细胞学技术的血样羊水栓塞的诊断方法,并对其有效性进行研究。方法收集羊水栓塞者血液样本,分别用两种直接涂片法(上清涂片法、沉淀涂片法)和两种液基细胞涂片法(自动涂片法、人工涂片法)对血液样本中的羊水成分进行检测,对4种方法的羊水成分检出率进行比较。结果建立的两种液基细胞涂片法的羊水成分检出率(分别为84.6%、92.3%)明显高于直接涂片法(分别为53.8%、61.5%)。结论液基细胞涂片法可以提高羊水栓塞的检出率。Objective To establish the diagnosis of am niotic fluid em bolismwith blood sam ples by liq-uid-based cytology technique and to study the validity of m ethod. Methods The blood sam ples were collected from patients who suffered from am niotic fluid em bolism. The com ponents of am niotic fluid in blood samples were examined with blood smear by two direct smear methods（supernatant smear, sedi-ment smear） and two liquid-based cytology methods（autom atic smear, manual smear）. The positive de-tection rate of each m ethod was calculated. Results The positive detection rates of two liquid-based cy-tology methods（84.6% and 92.3%, respectively） were m uch higher than those of two direct methods（53.8% and 61.5%, respectively）. Conclusion The liquid-based cytology technique could im prove the positive detection rate of am niotic fluid em bolism.

关 键 词：法医病理学 栓塞 羊水 液基细胞 

分 类 号：DF795.1[医药卫生—法医学]