心脑宁片对脾脏组织的影响  

Effect of Xinnaoning Tablets on Spleen Tissue

作　　者：吕宏迪[1] 王灵[1] 郝少君[1] 张正臣[1] 

机构地区：[1]中国人民解放军第371医院,河南新乡453000

出　　处：《中医学报》2014年第12期1778-1779,1782,共3页Acta Chinese Medicine

基　　金：河南省科技发展计划项目(编号:092102310202)

摘　　要：目的:观察心脑宁片对D-半乳糖亚急性衰老模型小鼠脾脏组织的影响。方法:72只小鼠随机分为6组,每组12只(雌雄各半),其中1组为空白对照组,每天颈背部皮下注射同体积生理盐水;另5组(模型组,脑安片组,心脑宁片大剂量组、中剂量组、小剂量组)制作D-半乳糖亚急性衰老模型,每天颈背部皮下注射D-半乳糖(生理盐水配成5%,注射量为0.025 m L·g-1)1次,连续40 d。从第11天开始,心脑宁大剂量组、中剂量组、小剂量组分别灌服心脑宁片混悬液(2.24 g·kg-1、1.12 g·kg-1、0.56 g·kg-1,112 g·L-1、56 g·L-1、28 g·L-1,0.02 m L·g-1,相当于临床剂量的30倍、15倍、7.5倍),脑安片组及模型组分别灌服脑安片混悬液(0.53 g·kg-1,26.5 g·L-1,0.02 m L·g-1,相当于临床用量的15倍)及同体积的生理盐水;空白对照组灌服同体积的生理盐水。于用药第40天,测量脾小结大小、淋巴细胞数,并光镜下观察脾脏组织形态的变化。结果:与模型组比较,心脑宁片大剂量组、中剂量组和脑安片组均可显著增加脾脏淋巴细胞数(P<0.01),对脾小结仅有增大趋势;以心脑宁片大剂量组、中剂量组对小鼠D-半乳糖衰老模型脾脏淋巴细胞数的增加作用为优。结论:心脑宁片对D-半乳糖亚急性衰老模型小鼠脾脏组织有较好的改善作用。Objective： To observe the effect of Xinnaoning tablets on D-galactose subacute aging model mice spleen tissue. Methods： 72 mice were randomly divided into 6 groups,12 rats in each group（ male and female）,of which one group as control group,daily subcutaneous injection of the same volume of physiological saline; the other 5 groups（ model group,Nao＇ an tablet group,large dose group of Xinnaoning piece,middle dose group,small dose group） production the subacute aging model induced by D-galactose,daily subcutaneous injection of D-galactose（ saline match 5%,injection volume was 0. 025 m L·g^- 1） the production of glucose metabolism in aging model,daily injection of one,continuous 40 days. From the beginning of the eleventh day,Xinnaoning large dose group,medium dose group,low dose group were intragastrically Xinnaoning tablets suspension（ 2. 24 g·kg^-1,1. 12 g·kg^-1,0. 56 g·kg^-1,112 g·L^-1,56 g·L^-1,28 g·L^-1,equivalent to the clinical doses of 30,15,7. 5 times）,Nao＇an tablets suspension（ 0. 53 g·kg^-1,26. 5 g·L^- 1,0. 02 m L·g^- 1,15 times equivalent to the clinical dosage） and saline of the same volume; blank control group saline perfusion of the same volume. On the fortieth day after medication,measuring the size of spleen nodules,lymphocyte count,and the changes were observed under the light microscope splenic tissue morphology. Results： compared with the model group,high dose group of Xinnaoning piece,middle dose group and Nao＇an tablets group significantly increased the number of spleen lymphocyte（ P〈0. 01）,on the splenic nodules,only increasing trend; Xinnaoning piece of high dose,middle dose group of lymphoid cells in the spleen of mouse D-galactose aging model to increase the number of role optimal. Conclusion： Xinnaoning tablets has the improving effect on D-galactose subacute aging model mice spleen tissue.

关 键 词：心脑宁片 脾脏组织 脑安片 小鼠 

分 类 号：R285.5[医药卫生—中药学]