紫外分光光度法测定蒙药广枣药材中总黄酮含量  

Determination of Total Flavones from Mongolia Medicine Choerospondias axillaris by UV-Spectrophotometry

作　　者：吴春芝[1] 李海华[2] 谷福根[1] 

机构地区：[1]内蒙古医科大学附属医院,内蒙古呼和浩特010050 [2]内蒙古医科大学,内蒙古呼和浩特010010

出　　处：《中医学报》2014年第12期1786-1788,共3页Acta Chinese Medicine

基　　金：内蒙古医科大学附属医院科研基金项目(编号:NYFYYB2010009)

摘　　要：目的：建立蒙药材广枣中总黄酮（TFC）的含量测定方法,为完善该药材的质量标准提供依据。方法：采用紫外分光光度法,以亚硝酸钠为还原剂,硝酸铝为络合剂,氢氧化钠为显色剂,在510 nm处测定TFC的吸光度值。结果：芦丁质量在0.2～1.2 mg范围内与吸光度呈良好线性关系（r=0.999 0）,平均回收率为101.15%,RSD为0.84%（n=9）。结论：本研究所建立广枣中TFC含量测定方法,操作简便,准确可靠,可用于该药材的质量评价。Objective： To establish a UV method for determining the content of TFC from Choerospondias axillaris so as to further provide an theoretical basis for improving the quality standards of the drug. Methods： A UV method was applied with sodium nitrite as a reductant,aluminum nitrate as complexing agent,sodium hydroxide as chromogenic agent,respectively. The absorbance of TFC was measured at 510 nm. Results： There was a good linear relationship between the absorbance and concentration of TFC ranging from 0. 2 ~ 1. 2 mg（ r = 0. 999 0） with the average recovery of 101. 15%,RSD was 0. 84%（ n = 9）. Conclusion： The established UV method is simple,accurate and reliable for controlling the quality of Choerospondias axillaris.

关 键 词：广枣 总黄酮 含量测定 紫外分光光度法 

分 类 号：R284.2[医药卫生—中药学]