HPLC法测定头孢噻吩钠含量的改进  

Improvement of Content Determination Method of Cefalotin Sodium by HPLC

作　　者：李玲玲[1] 周劲康 

机构地区：[1]广东省中医院珠海医院,广东珠海519000 [2]中山新意康医药科技有限公司,广东中山528400

出　　处：《今日药学》2014年第12期873-874,889,共3页Pharmacy Today

摘　　要：目的对HPLC法测定头孢噻吩钠含量的方法进行改进。方法选用Thermo BDS HYPERSIL C18（250 mm×4.6 mm,5μm）色谱柱,流动相为甲醇-0.2%磷酸（30∶70）,检测波长为254 nm;流速为1.0 m L/min。结果本品中头孢噻吩含量测定线性范围为2.002-20.020μg（r=1.000 0）,平均回收率为99.2%（RSD=0.4%,n=6）。结论该改进后的测定方法更快速、准确,重复性好;检测结果同《中国药典》（2010年版）方法所得的结果基本相同。Objective To improve the content determination method of the Cefalotin sodium by HPLC. Methods The Thermo BDS HYPERSIL C18（ 250 mm×4. 6 mm,5 μm） column was used,and the mobile phase was composed of Methanol-0. 2%Phosphoric acid solution（ 30 ∶ 70）,the detection wavelength was 254 nm; and the flow was 1. 0 m L / min. Result The method is suitable to determinate the content of Cefalotin sodium,the main peak and the other impurities peaks could be separated efficiently by this method; and Cefalotin showed a good linearity in the range of 2. 002-20. 020 μg（ r = 1. 0000）; and the average recovery was 99. 2% with RSD of 0. 4%（ n = 6）. Conclusion The improvement method is more simple,more accurate and reproducible,and the determination result of the method is as same as the described method in China Pharmacopoeia（ 2010）.

关 键 词：头孢噻吩钠 含量测定 HPLC 改进 

分 类 号：R927[医药卫生—药学]