ERK1/2通路抑制剂PD98059影响卵巢癌细胞SKOV3增殖、侵袭能力的研究  

Study on the effect of ERK1/2 pathway inhibitor PD98059 on proliferation and invasive ability of ovarian cancer SKOV3 cells

作　　者：吴忱莼 李留霞[1] 郭瑞霞[1] 雷婧[1] 李秀芳[1] 张孝艳[1] 张颖[1] 

机构地区：[1]郑州大学第一附属医院妇产科河南省临床重点开放实验室,河南郑州450052

出　　处：《中国妇幼保健》2015年第2期281-284,共4页Maternal and Child Health Care of China

基　　金：河南省教育厅重点项目〔No:13A320647〕;教育部新世纪优秀人才支持计划项目〔No:NCET-1000〕

摘　　要：目的:探讨MAPK/ERK信号传导通路抑制剂PD98059对卵巢癌细胞SKOV3的增殖、侵袭能力的影响。初步探讨阻断MAPK/ERK信号传导通路治疗卵巢癌的可能性。方法:体外培养人上皮性卵巢癌细胞SKOV3,采用CCK-8细胞增殖方法检测卵巢癌细胞SKOV3增殖情况,Western blot检测p-ERK蛋白表达,Transwell细胞侵袭实验检测SKOV3细胞的侵袭能力。结果:CCK-8法显示PD98059在一定范围内能够明显抑制卵巢癌细胞SKOV3的增殖,其作用具有时间依赖性及浓度依赖性,PD98059有效浓度为25μmol/L,在此有效浓度作用下有效时间为24h;Western blot法检测结果显示PD98059能够下调pERK1/2,即抑制ERK1/2磷酸化,从而使得ERK1/2信号传导途径失活;Transwell细胞侵袭实验结果显示PD98059在一定浓度范围内能抑制卵巢癌细胞SKOV3的侵袭与转移能力。结论:PD98059可以通过抑制ERK1/2信号途径,抑制人上皮性卵巢癌SKOV3细胞的增殖、侵袭转移。Objective：To explore the effect of MAPK/ERK signal transduction pathway inhibitor PD98059 on proliferation and inva- sive ability of ovarian cancer SKOV3 cells and the possibility of treatment of ovarian cancer by blocking MAPK/ERK signal transduction path- way. Methods ： Human ovarian cancer SKOV3 cells were cultured in vitro, the proliferation of ovarian cancer SKOV3 cells was detected by CCK8 assay, p - ERK expression was determined by Western blot, the invasive ability of ovarian cancer SKOV3 cells was detected by Tran- swell invasion trial. Results ： PD98059 inhibited the proliferation of ovarian cancer SKOV3 cells significantly within certain limits, showing a time - and concentration - dependent manner. The effective concentration of PD98059 was 25 μmoL/L, and the effective time was 24 hours; PD98059 down - regulated p - ERK1/2, and inhibited phosphorylation of ERK1/2, so ERK1/2 signal transduction pathway was inactivated; PD98059 could inhibit proliferation and invasive ability of ovarian cancer SKOV3 cells within a certain concentration range. Conclusion： PD98059 can inhibit proliferation, invasion and metastasis of human ovarian cancer SKOV3 cells by inhibiting ERK1/2 signal pathway.

关 键 词：ERK1/2信号通路 卵巢癌细胞 PD98059 P-ERK 细胞增殖实验 细胞侵袭实验 

分 类 号：R711.75[医药卫生—妇产科学]