荷斯坦奶牛β-氧化关键酶ECHS1基因甲基化分析  

Methylation Analysis of β-oxidation Key Enzyme ECHS1 Gene in Holstein Cows

作　　者：王凤武[1] 姜明德 付绍印[1] 李晓奇[1] 郭文华[1] 闫红霞[1] 

机构地区：[1]内蒙古农牧业科学院,内蒙古呼和浩特010031 [2]内蒙古通辽市科尔沁区兽医药械供应站,内蒙古通辽028000

出　　处：《畜牧与饲料科学》2014年第12期7-9,12,共4页Animal Husbandry and Feed Science

基　　金：内蒙古农牧业科学院创新基金项目(2011CXJJM05-5)

摘　　要：为了检测烯脂酰辅酶A水合酶短链1(ECHS1)的甲基化状态,以同源比对克隆的方法获得荷斯坦奶牛ECHS1部分片段的序列,首先预测甲基化数目,然后采用重亚硫酸盐转化测序(BS-Seq)的方法检测了所获得片段的甲基化状态。结果表明,该试验成功获得了ECHS1 459 bp的序列,其C+G含量为53.88%,含有28个Cp G岛,其中1号、2号、9号、11号、13号、14号、17号、22号、24号、27号位点所有个体都发生了甲基化。In order to detect the methylation status of enoyl-CoA hydratase short chain 1 （ECHS1）, the sequence fragments of ECHS1 was obtained for Holstein cows by homologous cloning. The number of methylation was firstly predicted, and then methylation status of the obtained sequence fragments was detected by method of sequencing of bisulfite conversion. The results showed that fragments of ECHS1 with the size of 459 bp were obtained. C＋G content was 53.88%. The fragments contained 28 CpGs islands and all individuals at sites 1, 2, 9, 11, 13, 14, 17, 22, 24 and 27 had been methylated.

关 键 词：烯脂酰辅酶A水合酶短链1 重亚硫酸盐转化测序 甲基化 

分 类 号：Q75[生物学—分子生物学] S823.91[农业科学—畜牧学]