有效分离甲型流感病毒方法的探讨  

Exploration for an effective method of isolating influenza A viruses

作　　者：杜溪乔[1] 任莉娜[1] 范荣军[1] 王宇[1] 怀清洁 梁爽[1] 

机构地区：[1]哈尔滨市疾病预防控制中心病毒检验科,黑龙江哈尔滨150056

出　　处：《中国卫生检验杂志》2014年第24期3532-3533,共2页Chinese Journal of Health Laboratory Technology

基　　金：黑龙江省卫生厅科研课题(2013310)

摘　　要：目的有效分离甲型流感病毒,提高病毒分离率。方法选择在2012年10月-2014月5月期间,经实时定量荧光PCR(real-time PCR)检测为甲型流感病毒阳性的咽拭样品,并采用MDCK细胞进行病毒分离;对血凝素(HA)滴度<8的一代培养物分别进行原倍、2倍、5倍、10倍稀释后进行传代培养,比较不同稀释度传代培养物血凝实验效果。结果 2倍稀释培养物传代培养后,血凝实验效果分别与其他3种进行比较,差异有统计学意义。结论甲型流感病毒分离中,对培养物进行2倍稀释后病毒分离效果最好。Objective To isolate influenza A virus effectively,increase the rate of virus isolation. Methods Throat swab samples which were detected as specifical influenza virus positive specimens by real- time PCR were isolated in MDCK cell between October 2012 and May 2014. After the original times,2 times,5 times and 10 times dilution subcultured these specimens whose the HA（ hemagglutination titer） was less than 8. Compare the HA results of all the diluted subculture. Results The experimental results of blood coagulation of 2 times diluted subculture were compared with others,and there are significant differences. Conclusion In the isolation of influenza A viruses,2 times diluted subculture was more effective.

关 键 词：狗肾传代细胞 流感病毒分离 血凝素滴度 倍比稀释 

分 类 号：R373.13[医药卫生—病原生物学]