MCP-1在结核病诊断中的价值  

Diagnostic value of MCP-1 for tuberculosis

作　　者：曹志红[1] 曹彦[1] 程小星[1] 

机构地区：[1]北京解放军第309医院全军结核病研究所,全军结核病防治重点实验室,100091

出　　处：《临床肺科杂志》2015年第2期195-198,共4页Journal of Clinical Pulmonary Medicine

基　　金：国家自然科学基金资助(No 81071318)

摘　　要：目的通过测定外周血单个核细胞(PBMCs)中单核细胞趋化蛋白(MCP-1)的表达水平,探讨其对结核诊断的意义。方法收集活动性肺结核患者、结核潜伏感染(latent tuberculosis infection,LTBI)及非结核感染健康对照个体的全血并提取PBMCs,经结核特异性抗原肽刺激后,采用基因芯片分析方法和酶联免疫吸附试验(ELISA)比较各组MCP-1表达情况。结果基因芯片分析发现活动期肺结核患者MCP-1表达(4460±3154)明显高于LTBI组(951.8±641.5,P=0.0043)。ELISA分析结果发现刺激前三组MCP-1浓度无差异(P=0.4747),而刺激后只有活动期肺结核患者组(37112±3790 pg/ml)和非结核感染健康对照组(15546±3143 pg/ml)之间的MCP-1浓度相比有显著差异(P<0.0001)。结论高表达的MCP-1有可能作为诊断结核及监测结核严重程度的指标之一,但是无法区分潜伏感染和活动期感染病例。Objective To evaluate the diagnostic value of MCP-1 for tuberculosis by assaying its expression level in PBMCs.Methods The peripheral blood was collected from pulmonary tuberculosis patients,latent tubercu-losis infection （LTBI）and non-tuberculosis infection healthy individuals.Then MCP-1 level in PBMCs stimulated with Mtb-specific antigens was detected using microarray analysis methods and enzyme-linked immunosorbent assay （ELISA）.Results Microarray analysis showed that MCP-1 level in patients with active tuberculosis （4460 ±31 54） was significantly higher than that in the LTBI group （951.8 ±641.5,P=0.0043）.ELISA analysis revealed no difference in MCP-1 concentration in PBMCs culture supernatants among the three groups before stimulation （P=0.4747）,but there was significant difference in MCP-1 concentration between the active tuberculosis patients group （371 1 2 ±3790 pg/ml）and the healthy control group （1 5546 ±31 43 pg/ml）after stimulation （P〈0.0001 ）.Con-clusion The high expression of MCP-1 can be used as an indicator for diagnosis of tuberculosis and monitor the dis-ease severity,but it can＇t distinguish latent infection from active infection.

关 键 词：结核 趋化因子 MCP-1 LTBI 

分 类 号：R52[医药卫生—内科学]