葡萄A病毒的IC-RT-PCR检测研究  

Study on Detection of Grapevine virus Aby Immunocapture Reverse Transcription-Polymerase Chain Reaction(IC-RT-PCR)

作　　者：魏梅生[1] 马洁[1] 李桂芬[1] 

机构地区：[1]中国检验检疫科学研究院植物检疫研究所,北京100029

出　　处：《北方园艺》2015年第1期99-103,共5页Northern Horticulture

基　　金：国家质量监督检验检疫总局课题资助项目(2012IK302)

摘　　要：葡萄A病毒是葡萄皱木综合证的病原之一,通常与其它病毒混合发生,造成葡萄产量的损失。为有效检测葡萄A病毒,该研究建立了免疫捕获-反转录-聚合酶链式反应检测方法(IC-RT-PCR),将免疫学和核酸扩增技术结合起来使用。结果表明:IC-RT-PCR方法检测的特异性较强,检测葡萄A病毒2个株系的结果为阳性,检测葡萄扇叶病毒、南芥菜花叶病毒、烟草环斑病毒结果为阴性。该方法检测提纯葡萄A病毒的灵敏度为10ng/mL,检测葡萄A病毒感染的Nicotiana benthamiana叶片可稀释到10-6。Grapevine virus A （GVA） is one of pathogens causing grapevine rugose wood complex, which can be caused by many kinds of viruses simultaneously. In order to more effectively detecting this virus, an immunocapture reverse transcription-polymerase chain reaction （IC-RT-PCR） was developed for detecting （;rapevine virus A in this study. The results showed that 2 strains of GVA were detected using this method. There was no cross reaction with Grapevine fanleaf virus ,Arabis mosaic virus and Tobacco ringspot virus. The sensitivity of the assay was 10 ng/mL for detecting purified GVA. Extract from GVA infected Nicotiana benthamiana leaves was detected to a dilution of 10-6.

关 键 词：葡萄A病毒 IC-RT-PCR 

分 类 号：S436.631[农业科学—农业昆虫与害虫防治]