蓝莓茎段离体快繁研究  

Study on Rapid Proliferation of Stem Segments in vitro of Blueberry

作　　者：赵兴宇[1] 李丽丽[2,3] 杨洪一[1] 

机构地区：[1]东北林业大学生命科学学院,黑龙江哈尔滨150040 [2]黑龙江省林业科学研究所,黑龙江哈尔滨150081 [3]黑龙江省农业科学院耕作栽培研究所,黑龙江哈尔滨150086

出　　处：《北方园艺》2015年第1期103-105,共3页Northern Horticulture

基　　金：国家自然科学基金资助项目(31200517;31300573);中国博士后科学基金资助项目(2011M500706;2012T50382)

摘　　要：以大兴安岭地区野生蓝莓苗木为试材,取当年生幼嫩茎段为外植体,探索建立蓝莓组培苗的快繁体系。结果表明：外植体消毒处理组合为75%乙醇处理10-15s,0.1%升汞处理10min。培养基为WPM培养基,愈伤组织诱导及不定芽增殖时期应用的激素组合ZT 2.0mg/L＋NAA 0.2mg/L。利用IBA蘸根法诱导不定根,激素浓度为200μg/mL,芽诱导率超过80%,生根率超过70%。Ihking the wild blueberry seedlings in Greater hinggan mountains area as test materials,using the young stem segments as explants,the blueberry somaclone of rapid propagation system was explored. The results showed the explants disinfection treatment combination was 75% ethanol for 10--15 seconds and 0. 1% mercuric chloride for 10 min. The medium was WPM,and the hormones were ZT 2. 0 mg/L＋NAA 0. 2 mg/mL in the steps of inducement of callus and proliferation of adventitious buds. Adventitious roots were induced with dipping method hy 200 μg/ml. IBA. The results showed that callus and adventitious buds could be formed from stem of blueberry by the medium and hormones,and the induced ratio of adventitious buds was higher than 80%. In addition,the rooting rate was more than 70%.

关 键 词：蓝莓 外植体 组织培养 不定根 

分 类 号：S663.203.6[农业科学—果树学]