新疆维吾尔族、汉族结直肠癌患者中KRAS及BRAF基因突变差异及其与预后的关系研究  

Prognostic in role of KRAS and BRAF in colorectal cancer cases with synchronous liver metastases in the Uyghur and Han nationality in Xinjiang

作　　者：孙毅[1] 艾力江.吐尔逊 杨娜[2] 古丽比也.沙比尔 赵化荣[1] 王文然[1] 刘攀[1] 肖蕾[1] 玛依努尔.艾力 包永星[1] 

机构地区：[1]新疆医科大学第一附属医院肿瘤中心,乌鲁木齐830054 [2]新疆维吾尔自治区人民医院放疗一科,乌鲁木齐830001

出　　处：《新疆医科大学学报》2015年第1期47-51,共5页Journal of Xinjiang Medical University

基　　金：新疆医科大学第一附属医院科研奖励基金(2011YFY11);吴阶平医学基金(320.6799.1143);乌鲁木齐市科技技术计划项目乌市感染与肿瘤重点实验室(H111313001)

摘　　要：目的探讨新疆维吾尔族(维族)、汉族结直肠癌伴肝转移患者中KRAS基因及BRAF基因突变差异及其与临床病理特征和预后的关系。方法选择新疆医科大学第一附属医院2005年1月-2010年12月收治的100例新疆维、汉族结直肠癌伴肝转移患者,应用PCR及Pyrosequencing测序法检测分析KRAS基因及BRAF基因突变情况,分析其与临床病理特征和预后的关系。结果维、汉族结直肠癌伴肝转移患者的KRAS与BRAF基因突变率分别为28%(28/100)与5%(5/100)。50例汉族患者中KRAS基因突变18例,BRAF基因突变2例;50例维吾尔族患者中KRAS基因突变10例,BRAF基因突变3例。原发肿瘤组织学分级、区域淋巴结转移、脉管瘤栓、肝外侵犯或转移、KRAS和BRAF为结直肠癌的预后影响因素(P<0.05);多因素分析显示,脉管瘤栓(P=0.035)、KRAS基因(P=0.001)为结直肠癌的独立的预后因素。结论在新疆维、汉族结直肠癌伴肝转移患者中,脉管瘤栓及KRAS基因具有主要的预后价值。积极检测KRAS基因,可望延长患者生存期。Objective To analyse the relations among clinical pathological characteristics,prognosis and gene mutation of KRAS and BRAF with whom carry Colorectal Cancer Cases and Synchronous Liver Me-tastases in the uyghur and han nationality in Xinj iang.Methods The clinical and pathological data of Colo-rectal Cancer Cases with Synchronous Liver Metastases in the Uyghur and han nationality in Xinj iang trea-ted at Xinjiang MedicaI University from January 2005 to December 2010 were retrospectively reviewed and analyzed,including KRAS and BRAF.Results KRAS and BRAF tumor mutation rates were 28.0% and 5.0%,respectively.Of 50 cases Han nationality ,18 cases KRAS and 2 cases BRAF were gene multation, of the uyghur,10 cases KRAF and 3 cases BRAF.Univariate analysis with Kaplan-Meier methods revealed that the differentiation of the primary tumor,N status,lymphatic and vascular invasion,extrahepatic disease, KRAS and BRAF were prognostic factors （P＆lt;0.05）.Multivariate analysis showed that lymphatic and vascular in-vasion （P=0.035）,KRAS （P =0.001）were independent prognostic factors of colorectal cancer patients with 〈br〉 synchronous liver metastases.Conclusion The lymphatic and vascular invasion and KRAS have significant prog-nostic value for colorectal cancer with synchronous Iiver metastases.It is necessary to detect KRAS gene for Color-ectal Cancer Cases with Synchronous Liver Metastases in the uyghur and han nationality in Xinj iang.

关 键 词：新疆 维吾尔族 汉族 结直肠癌 同时性肝转移 预后 

分 类 号：R657.1[医药卫生—外科学]