动物源性食品中红霉素残留的ELISA检测  

Determination of Erythromycin Residues in Animal-derived Food by Enzyme-linked Immunosorbent Assay

作　　者：崔廷婷 钟建三 张瑜 杨昌松 白莉 

机构地区：[1]北京勤邦生物技术有限公司,北京102206 [2]湖南红星盛业食品股份有限公司,湖南长沙410004

出　　处：《四川畜牧兽医》2015年第1期29-31,共3页Sichuan Animal & Veterinary Sciences

基　　金：十二五"国家科技支撑计划项目<食品质量安全控制及检测关键技术与产品>之课题"水产品加工过程质量安全控制关键技术集成与示范(2012BAD29B06)

摘　　要：本试验采用间接竞争酶联免疫分析法（ELISA法）测定了动物源性食品中红霉素的残留,结果显示：猪肉、猪肝、牛肉、鱼肉、虾肉、蜂蜜和牛奶中的红霉素检测限分别为4.13、8.23、0.78、3.75、7.94、3.09μg/kg和1.87μg/L,样品添加回收率范围为69.0%-107.5%,批内变异系数为5.7%-11.4%,批间变异系数为8.7%-14.0%;与硫氰酸红霉素的交叉反应率为114%,与琥乙红霉素的交叉反应率为67.4%,与其他药物的交叉反应率均小于0.1%。试验表明,采用ELISA法测定红霉素准确灵敏、重复性好、特异性高,适用于检测动物源性食品中的红霉素残留。An indirect competitive enzyme-linked immunosorbent assay （ELISA） was used to measure the residues of erythromycin in animal-derived food. The results showed that limits of detection in pork, pork liver, beef, fish, shrimp, honey and milk were 4.13, 8.23, 0.78, 3.75, 7.94, 3.09μg/kg and 1.87μg/L respectively. Sample recoveries were 69.0%-107.5%, inter-assay coefficient variations were 5.7%-11.4%, and intra-assay coefficient variations were 8.7%-14.0%. The cross-reactivity towards erythromycin thiocyanate and erythromyein ethylsuccinate were 114% and 67.4% respectively. As for other drugs, the cross-reactivity was less than 0.1%. In a word, the sensitivity, accuracy, repeata- bility and specificity of ELISA method were satisfactory, it was suitable for measuring the residues of erythromycin in animal-derived food.

关 键 词：红霉素 残留物 酶联免疫法 动物源性食品 

分 类 号：S859.84[农业科学—临床兽医学]