天冬氨酸对脂多糖刺激仔猪结肠黏膜结构和屏障功能的影响  

Effect of Aspartate on Colonic Morphology and Barrier Function in Piglets with Lipopolysaccharide Challenge

作　　者：刘称[1] 朱惠玲[1] 刘玉兰[1] 皮定安[1] 冷炜博[1] 王秀英[1] 吴欢听 侯永清[1] 

机构地区：[1]武汉轻工大学动物营养与饲料科学湖北省重点实验室,湖北武汉430023

出　　处：《中国畜牧杂志》2015年第1期38-42,共5页Chinese Journal of Animal Science

基　　金：教育部新世纪优秀人才支持计划(NCET-10-0158);国家自然科学基金面上项目(31172222;31372318);湖北省自然科学基金杰出青年人才项目(2013CFA029);湖北省国际科技合作项目(2011BFA008)

摘　　要：本试验旨在研究天冬氨酸(ASP)对脂多糖(LPS)刺激仔猪结肠黏膜结构和屏障功能的影响。试验选用24头仔猪,4个处理组分别为对照组、LPS组、0.5%ASP组、1.0%ASP组。试验第19天,分别给LPS组、0.5%ASP组、1.0%ASP组试验猪注射100μg/kg体重的LPS,对照组注射等量的生理盐水。注射后24 h屠宰,取结肠样品待测。结果表明:对照组仔猪结肠形态结构完整;注射LPS后,结肠上皮部分脱落;ASP组轻度脱落,与LPS组相比,0.5%ASP组结肠隐窝深度显著升高(P<0.05);0.5%和1.0%ASP可显著缓解LPS刺激导致的结肠杯状细胞数目的增多(P<0.05);0.5%ASP具有提高结肠中性粒细胞密度的趋势(P<0.10)。0.5%和1.0%ASP可显著缓解LPS刺激导致的结肠和血浆二胺氧化酶活性的降低(P<0.05)。以上研究结果表明,日粮添加ASP可有效阻止LPS对结肠结构和屏障功能的损伤。The study was conducted to evaluate the effects of aspartate （ASP） supplementation on colonic morphology and mucosal barrier function in piglets with lipopolysaccharide （LPS） challenge. Twenty-four piglets were randomly assigned into 4 groups： control; LPS; 0.5% ASP; 1.0% ASP. The trial period was 19 days. On day 19, the piglets in the LPS group, 0.5% ASP group and 1.0% ASP group were injected with 100 μg/kg BW LPS, and the piglets in the control group were injected with the same dose of sterile saline. After 24 h saline or LPS injection, all pigs were sacrificed to obtain colonic samples for analysis. The results showed that： the colonic morphology from piglets in the control group was intact. Colonic epithelium falled off in LPS group; Colonic epithelium in ASP group falled off to less degree. Compared with the LPS group, colonic crypt depth of 0.5%ASP was significantly increased （P〈0.05）. 0.5% or 1.0% ASP alleviated the elevation of goblet cell number induced by LPS challenge （P〈0.05）. 0.5% ASP tended to increase the density of colon neutrophils （P〈0.10）. 0.5% or 1.0% ASP alleviated the decrease of DAO activity induced by LPS challenge （P〈0.05）. Theses results indicate that ASP supplementation can prevent injury of colonic morphology and barrier function induced by LPS challenge.

关 键 词：天冬氨酸 脂多糖 结肠黏膜 屏障功能 仔猪 

分 类 号：S828.5[农业科学—畜牧学]