清脑复神液提取工艺再评价  

Re-evaluation on Extraction Process of Qingnao Fushen Liquids

作　　者：伍利华[1] 黄英 刘婷[3] 聂忠莉[3] 徐玉玲[4] 邹鹏[3] 刘涛[3] 

机构地区：[1]广西中医药大学,南宁530001 [2]四川新中方医药科技股份有限公司,四川广元628000 [3]成都大学,成都610106 [4]南京中医药大学,南京210046

出　　处：《中国实验方剂学杂志》2015年第1期10-13,共4页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：四川省经信委创新能力提升专项(2013NL021)

摘　　要：目的:对清脑复神液提取工艺进行再评价研究,为改进该制剂的生产工业提供实验依据。方法:采用HPLC测定黄芩苷含量,流动相甲醇-0.37%磷酸(47∶53),检测波长280 nm。以干膏量和黄芩苷提取量的综合评分为指标,通过正交试验考察溶媒用量、渗漉速度、乙醇体积分数对清脑复神液渗漉工艺的影响,利用紫外全波长扫描、近红外扫描和红外扫描比较不同提取液的理化性质。结果:最佳提取工艺为加8倍量40%乙醇于2.27 m L·min-1·kg-1渗漉;黄芩苷提取量分别为31.23 mg,干膏量21.64 g。渗漉法与浸渍法所得药液的干膏量及紫外全波长扫描、近红外扫描、红外扫描图谱均基本一致。结论:清脑复神液的渗漉工艺与原浸渍工艺提取的有效成分基本一致,可用渗漉工艺替代浸渍工艺。Objective： To study on re-evaluation of extraction process of Qingnao Fushen liquids, to provide experimental basis for improving industrial production of this preparation. Method： Determination of baicalin by HPLC, mobile phase of methanol-0. 37% phosphoric acid （47： 53） , detection wavelength of 280 nm. Taking composite score of amounts of dry extract and baicalin as indexes, orthogonal test was adopted to optimize percolation process with the amount of solvent, percolation velocity, ethanol concentration as factors. Physical and chemical properties of different extracts were compared through ultraviolet scanning, near infrared scanning and infrared scanning. Result： Optimum extraction condition was as following： percolated by 8 times the amount of 40% ethanol at 2.27 mL·min-1·kg-1; amounts of dry extract and baicalin were 21.64 g and 31.23 mg. Ultraviolet full wavelength scanning, near infrared scanning, infrared scanning and dry extract amount of percolate and immersion fluid were most the same. Conclusion： Effective ingredients extracted from these two kinds of extraction process are all most the same, so percolation process can substitute immersion method.

关 键 词：清脑复神液 浸渍法 渗漉法 黄芩苷 

分 类 号：R283.6[医药卫生—中药学] R284.2[医药卫生—中医学]