瑶族药风湿骨痛外擦酒的提取工艺和质量标准  

Extraction Technology and Quality Standard for Rheumatism Ostalgia Alcohols

作　　者：游燕[1] 庞宇舟[2] 潘智[1] 黄丁英[1] 刘圆圆[1] 易春霞[1] 

机构地区：[1]桂林市中医医院,广西桂林541002 [2]广西中医药大学,南宁530001

出　　处：《中国实验方剂学杂志》2015年第1期17-20,共4页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：广西壮族自治区卫生厅中医药制剂类课题(GZYZ-10-33)

摘　　要：目的：优选风湿骨痛外擦酒的提取工艺并制定其质量标准.方法：采用HPLC测定姜黄素含量,流动相乙腈-4％冰乙酸溶液（48∶52）,检测波长430 nm.以总固体量和姜黄素提取量的综合评分为指标,通过正交试验考察浸泡时间、乙醇体积分数及用量对风湿骨痛外擦酒提取工艺的影响.采用TLC对方中当归、血风藤、姜黄、香加皮进行定性鉴别.结果：姜黄素在0.021 2～0.106 μg线性关系良好（r=0.999 8）,平均回收率97.81％.最佳提取工艺为加10倍50％乙醇浸泡30 d.TLC分离效果好,斑点清晰,阴性对照无干扰.结论：该工艺稳定可行、指标成分提取量高,适用于风湿骨痛外擦酒的生产.建立的TLC能有效控制该产品质量.Objective： To optimize extraction technology of rheumatism ostalgia alcohols and establish its quality standard. Method： HPLC was adopted to determine the content of curcumin with mobile phase consisting of 4% glacial acetic acid-acetonitrile （52： 48） and detection wavelength at 430 nm. With composite score of amounts of general solid and curcumin as index, orthogoual design was employed to optimize extraction technology of rheumatism ostalgia alcohols with soaking time, ethanol concentration and amount as factors. Angelicae Sinensis Radix, Ventilago Leiocarpae Radix et Caulis, Curcumae Longae Rhizoma and Acanthopanacis Cortex were identified by TLC. Result： Curcumin showed a good linear relationship at a range of 0. 021 2-0. 106 μg with r of 0. 999 8 and average recovery of 97.81%. Optimal extracting technology was： soaked 30 days with 10 times the amount of 50% ethanol. TLC spots were clear and the blank test showed no interference. Conclusion： This technology is stable feasible with high extraction amount of index ingredient, which is available for industrial production of rheumatism ostalgia alcohols. This established TLC can control quality of this preparation effectively.

关 键 词：瑶族药 风湿骨痛外擦酒 姜黄素 质量标准 

分 类 号：R283.6[医药卫生—中药学] R284.2[医药卫生—中医学]