HPLC-Q-TOF-MS分析附子的化学成分及煎煮过程中的变化规律  

Chemical Constituents of Aconite Lateralis Radix and Changing Regularities During Decoction Process by HPLC-Q-TOF-MS

作　　者：黄志芳[1] 唐小龙[1,2] 罗恒[1] 张意涵[1,2] 刘云华[1] 陈燕[1] 刘玉红[1] 易进海[1] 

机构地区：[1]四川省中医药科学院,成都610041 [2]成都中医药大学,成都611137

出　　处：《中国实验方剂学杂志》2015年第1期57-63,共7页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：国家重点基础研究发展计划(973计划)项目(2009CB522804);国家科技支撑计划项目(2011BAI13B05)

摘　　要：目的：对附子生物碱进行分析鉴定并探究其主要成分在煎煮过程中的动态变化规律.方法：采用HPLC-Q-TOF-MS分析鉴定附子生物碱成分,基于溶出率分析附子主要成分在煎煮过程中的动态变化.结果：分析鉴定了附子中的36个生物碱成分,并测定了其中25个主要成分在煎煮过程中的溶出变化.双酯型生物碱（新乌头碱、乌头碱、次乌头碱、脱氧乌头碱等）迅速降低,约2h几乎检不出;单酯型生物碱（苯甲酰新乌头原碱、苯甲酰乌头原碱、苯甲酰次乌头原碱、10-羟基苯甲酰新乌头原碱、苯甲酰去氧乌头原碱、脱水苯甲酰次乌头原碱等）先逐渐升高,6～8h达到峰值,后逐渐降低;酯型原碱（新乌头原碱、乌头原碱、次乌头原碱）一直持续升高;其他原碱先逐渐升高,6～8h达到峰值,后趋于稳定.结论：对附子生物碱成分进行了较全面的分析,揭示了附子煎煮过程中各类生物碱含量的动态变化规律,为附子质量控制和临床应用提供参考.Objective： To identify the alkaloid compounds of Aconite Lateralis Radix and to probe into the dynamic changing regularities of main constituents during decoction process. Method： HPLC-Q-TOF-MS was used to identify chemical constituents and to analysis the content changes of main constituents during decoction process using dissolution rate. Result： Totally 36 alkaloid compounds of Aconite Lateralis Radix were identified and the dissolution rates of 25 compounds were determined in the process of decoction. The results showed that diester alkaloids （mesaconitine, hypaeonitine, aconitine and deoxyaconitine ） decreased quickly and their content were not detected after decocting 2 h; monoester alkaloids （benzoylaconine, benzoylmeaconine, benzoylhypaconine, 10-OH benzoylmeacinine, benzoyldeoxyaconine and dehydrated-benzoylhypaconine） firstly increased and reached the peak in 6 to 8 h and then gradually deceased. Aconine alkaloids （mesaconine, aconine and hypaconine） increased all the time during decoction process. General alkaloids firstly increased and reached to the peak in 6 to 8 h and then tended to stability. Conclusion： The analysis of the alkaloid compounds of Aconite Lateralis Radix was comparatively comprehensive and the dynamic changing regularities of the alkaloid compounds were explored. It provided a reference for quality control and clinic application of Aconite lateralis Radix.

关 键 词：附子 生物碱 高效液相色谱-四级杆飞行时间质谱联用 成分分析 煎煮 变化规律 

分 类 号：R284.1[医药卫生—中药学]