近红外漫反射光谱法快速测定枳实药材中醇浸出物的含量  

Rapid Quantitative Determination of Ethanol-extract in Aurantii Fructus Immaturus by Near-infrared Spectroscopy

作　　者：白雁[1] 樊明月[1] 雷敬卫[1] 谢彩侠[1] 张强[1] 

机构地区：[1]河南中医学院,郑州450046

出　　处：《中国实验方剂学杂志》2015年第1期73-76,共4页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：河南省教育厅科学技术研究重点项目(14B360007);河南省中药开发工程技术研究中心项目(142102313109);河南中医学院科技创新人才项目(2014XCXRC03)

摘　　要：目的:采用近红外漫反射光谱技术结合偏最小二乘法(PLS)建立一种枳实药材中醇浸出物含量的快速测定方法。方法:运用近红外漫反射光谱技术采集枳实的近红外漫反射光谱,以热浸法测定的含量为参考值,结合PLS建立枳实药材中醇浸出物含量的定量分析模型,并用未知样品验证该模型。结果:所建醇浸出物定量分析模型的校正集内部交叉验证相关系数(R2)、内部交叉验证均方差(RMSECV)和校正均方差(RMSEC)分别为0.984 89,0.417 89和0.153;验证集预测相关系数(r)与预测均方差(RMSEP)分别为0.948 4和0.188。结论:该方法操作简便,测定快速,结果准确,可用于枳实药材中醇浸出物含量的快速测定。Objective： This paper reported the application of near infrared spectroscopy with PLS for rapid quantitative determination of ethanol-extract in Aurantii Fructus Immaturus. Method： Through collecting near-infrared spectra of Aurantii Fructus Immatnrus by near-infrared diffuse reflectance spectroscopy technique, with hot-dip method measurements as a reference value, the quantitative model of ethanol-extract was built by partial least squares （PLS） , and it was verified by the unknown samples. Result： The correlation coefficients （R2）, root mean square error of cross validation （RMSECV）, and the root mean-square error of calibration （RMSEC） of the quantitative calibration model of ethanol-extract was 0. 984 89, 0.417 89 and 0. 153, respectively; the correlation coefficients of predication （r） and the root-mean-square error of predication （RMSEP） was 0.948 4 and 0. 188, respectively. Conclusion： The method is simple, quick, accurate and pollution-free, and it can be applied for the rapid determination of ethanol-extract in Aurantii Fructus Immaturus.

关 键 词：近红外光谱技术 偏最小二乘法 枳实 醇浸出物 快速测定 

分 类 号：R284.1[医药卫生—中药学]