降粘胶囊HPLC指纹图谱分析  

HPLC Fingerprint of Jiangnian Capsule

作　　者：张前飞[1] 杨玉琴[1] 秦利芬[2] 张丽艳[1] 赵成元 赵勇 苟正雷 

机构地区：[1]贵阳中医学院,贵阳550002 [2]云南省昆明市儿童医院,昆明650034 [3]贵州省遵义市人民医院,贵州遵义563000

出　　处：《中国实验方剂学杂志》2015年第1期77-81,共5页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：贵州省中药现代化科技产业研究开发专项([2010]5015号;ZY[2012]3008号)

摘　　要：目的：建立降粘胶囊的HPLC指纹图谱,为降粘胶囊的质量控制提供新的方法.方法：采用Agilent C18色谱柱（4.6 mm×250 mm,5μm）,流动相乙腈-0.05％磷酸水溶液梯度洗脱,柱温25℃,流速1 mL＆#183; min-1,进样量10μL,波长272 nm;运用国家药典委员会“中药色谱指纹图谱相似度评价系统软件A版”对10批降粘胶囊复方制剂进行相似度评价,并对复方中12味药材进行相关性分析.结果：建立了降粘胶囊指纹图谱,10批复方制剂相似度在0.97 ～ 0.99之间,以葛根素为参照峰,标记了28个共有峰,其中23个归属到各药材,4个已确认成分.结论：该方法具有良好的精密度、重复性、稳定性,可用于降粘胶囊的质量控制.Objective： To establish an HPLC fingerprint method for quality control of Jiangnian capsule. Method： The HPLC separation was performed on an Agilent ClS （4.6 mm ×250 mm,5μ） , using acetonitrile- 0.05% H3PQ water solution as mobile phase with flow rate of 1.0 mL·min-1 The detection wavelength was 272 nm. Column temperature was set at 35 ℃. Injection volume was 10 μL. The ＇ Similarity Evaluation System for Chromatographic Fingerprint of TCM＇ （2004 A edition） was used to evaluate the similarity of the 10 batches of Jiangnian capsule, and to analyze the correlations of 12 ingredients in Jiangnian capsule. Result： The similarity values of 10 batches Jiangnian capsule were between 0.97-0.99. Taking puerarin as the reference peak, 28 common peaks were found, of which 23 peaks were assigned to herbs, and 4 peaks were identified. Conclusion： The method has good precision, repeatability, stability, and can be used in the comprehensive quality evaluation of Jiangnian capsule.

关 键 词：降粘胶囊 指纹图谱 相似度 高效液相色谱法 

分 类 号：R284.1[医药卫生—中药学]