舒肝解郁胶囊对老年痴呆小鼠学习记忆能力的改善作用  

Study of Shugan Jieyu Capsule Improving Learning and Memory Ability on AD Model Mice

作　　者：吴建明[1,2,3] 马娜 张鸿程 柯潇 郝晓锋 张志荣[2] 高小平 

机构地区：[1]成都康弘药业集团博士后工作站,成都610036 [2]四川大学华西药学院博士后流动站,成都610041 [3]泸州医学院药学院,四川泸州646000

出　　处：《中国实验方剂学杂志》2015年第1期126-129,共4页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：四川省科技成果转化专项(2012SC0029);四川省重大产业技术创新专项(2012CD00082)

摘　　要：目的:观察舒肝解郁胶囊(Shugan Jieyu capsule,SGJY)对老年痴呆(Alzheimer's disease,AD)模型小鼠学习记忆能力的影响。方法:72只KM小鼠随机分为6组,即正常组、模型组、石杉碱甲组(0.07 mg·kg-1)及SGJY高、中、低剂量组(480,240,120 mg·kg-1)。各组每天均灌胃给药一次,给药体积为20 m L·kg-1,连续14 d。末次给药1 h后除正常组外均ip3 mg·kg-1氢溴酸东莨菪碱,20 min后进行水迷宫测试,收集血清及全脑并测定其血清及脑组织中丙二醛(malondialdehyde,MDA)及总抗氧化能力(total antioxidant capacity,T-AOC)水平。另取10只人鼠淀粉样前蛋白(APPswe)阴性转基因小鼠为正常组,50只APPswe阳性转基因小鼠随机分为5组,分组及剂量同前,连续灌胃给药30 d,末次给药1 h后进行跳台潜伏期与水迷宫测试(逃避潜伏期及游泳路径)。结果:与正常组比较,两种模型组小鼠逃避潜伏期及游泳路径均显著高于正常组(P<0.05,P<0.01);与模型组比较,SGJY高、中、低剂量组均可不同程度地降低跳台实验逃离电击潜伏期及Morris水迷宫实验到达安全平台的潜伏期及总路程,尤其以高、中剂量组最为显著(P<0.01),且呈明显量效关系。同时3个剂量组均能显著改善脑组织中的MDA及T-AOC水平(P<0.01)。结论:舒肝解郁胶囊可显著改善老年痴呆小鼠学习记忆能力。Objective： To investigate the influence of Shugan Jieyu capsule （SGJY） on learning and memory ability in model mice with Alzheimer＇s disease （AD）. Method： Seventy-two Kunming （KM） mice were randomly divided into six groups such as normal group, model group, huperzine A （ Hup A） group （0.07 mg ·kg-1） and SGJY groups （480, 240, 120 mg·kg-1 ）. The mice were gavaged once a day for 14 days, then induced by scopolamine hydrobromide （ip, 3 mg·kg-1） except normal group. The effect of SGJY was studied via Morris water maze test and the levels of malondialdehyde （MDA） and total antioxidant capacity （T-AOC） in plasma and brain tissue were detected, Randomly selected 10 non-APPswe transgenic mice were as normal group, other 50 APPswe transgenic mice with high expression of presenilin （PS） and amyloid precusor protein （APP） were divided into five groups as above, the mice were gavaged once a day for 30 days, then the effect of SGJY on learning and memory ability was evaluated by step-down test and water maze test. Result： Compared with normal groups, the levels of latencies and total distances before escaping onto the security platform increased significantly （P〈0, 05, P 〈0.01 ）. Compared with model groups, SGJY groups in various dosage （480, 240, 120 mg·kg-1） could shorten the latency period to escape from electric shock. In Morris water maze test, three groups of SGJY could shorten latencies and total distances before escaping onto the security platform at different levels. The effectwas the most obvious in the group of high and middle dose SGJY relationship. Meanwhile, we also found that the SGJY of three different of MDA and increase that of T-AOC in brain tissue （P 〈 0.01）. improving the learning and memory abilities of AD model mice. （ P 〈 0.01 ） , which showed dose-effect doses could significantly decrease the levels Conclusion： SGJY has marked effect of

关 键 词：舒肝解郁胶囊 老年痴呆 东莨菪碱 APPswe转基因小鼠 

分 类 号：R285.5[医药卫生—中药学]