益肺通络方对低氧性肺血管重建大鼠肌型肺小动脉中膜增殖的影响  

Effect of Yifei Tongluo Formula on Muscular Arteries Medial Smooth Muscle Cell Proliferation of Hypoxiainduced Pulmonary Vascular Remodeling in Rats

作　　者：刘旻[1] 刘贵颖[1] 胡仁杰 冯文茹 李翀[1] 朱振刚[1] 沈洪昇 

机构地区：[1]天津中医药大学第一附属医院,天津300193 [2]天津医药科技研究所,天津300020

出　　处：《中国实验方剂学杂志》2015年第1期140-144,共5页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：天津市卫生局中医;中西医结合科研专项资金课题(07066)

摘　　要：目的:观察益肺通络方对低氧性肺血管构型重建(PVR)大鼠肌型肺小动脉中膜增殖的影响。方法:60只Wistar大鼠随机分成空白、模型、益肺通络方高、中、低剂量5组,每组12只,采用气管注入0.2 m L脂多糖(1 g·L-1)联合烟熏(每次5支,30 min/次)4周建立慢性阻塞性肺疾病(COPD)大鼠模型,叠加每天吸入18%低氧2周建立肺血管构型重建(PVR)大鼠模型。空白、模型组生理盐水5 m L·d-1天灌胃,实验组按生药浓度分高剂量(30 g·kg-1),中剂量(20 g·kg-1),低剂量(10 g·kg-1)益肺通络方煎剂浓缩液5 m L·d-1灌胃。4,5,6周3个时点取材,测量中膜厚度、血管管径和中膜面积、血管面积,计算相对中膜厚度/面积。结果:相对中膜厚度/面积比较,4周时,模型组>实验低剂量组>实验中剂量组>实验高剂量>空白组,组间差异均有统计学意义(P<0.05)。5周时,模型组>实验低剂量组>实验中剂量组>实验高剂量>空白组,组间差异均有统计学意义(P<0.05)。6周时,模型组>实验低剂量组>实验中剂量组>实验高剂量>空白组,组间差异均有统计学意义(P<0.05)。结论:益肺通络方能抑制慢性低氧PVR大鼠肌型肺小动脉中膜增殖,且存在量效关系。Objective ： To observe the effect of Yifei Tongluo formula on muscular arteries medial smooth muscle cell proliferation of hypoxia-induced pulmonary vascular remodeling in rat. Method： Sixty Wistar rats were divided into 5 groups： blank group, model group, experimental group （high-does, middle-does, low-does） , 12 rats in each group. The trachea injection of 0.2 mL lipopolysaccharide （1 g·L-1） combined smoke method for four weeks was adopted to establish the model of chronic obstructive pulmonary disease （COPD）, and superposition of low oxygen （18%） two weeks establish pulmonary vascular remodeling （PVR） models in rats. The blank and model groups were perfused with saline, experimental group was perfused with Yifei Tongluo formula （30, 20, 10 g·L-1）. Samples were collected in 4, 5, 6 weeks respectively. The middle membrane thickness, vascular diameter and middle membrane area, vascular area were measured, then calculated the ratio of relative middle membrane thickness and area. Result： The ratio of relative middle membrane thickness and area in each point-in-time between the groups showed t as follows ： model group 〉 low-does group 〉 middle-does 〉 high-does 〉 blank group, with significant difference. Conclusion： Yifei Tongluo formula can inhibit the muscular arteries medial smooth muscle cell proliferation of hypoxia-induced pulmonary vascular remodeling in rats.

关 键 词：肺血管构型重建 血管平滑肌增殖 益肺通络方 

分 类 号：R285.5[医药卫生—中药学]