辛弗林对豚鼠离体气管平滑肌的作用及机制  

Effects and Mechanism of Synephrine on Contraction of Isolated Guinea-pig Tracheal Smooth Muscle

作　　者：魏莹[1,2] 裴昆[1] 陈海芳[1] 罗小泉[1] 骆利平[1] 易徐航 董晶[1] 杨武亮[1] 

机构地区：[1]江西中医药大学现代中药制剂教育部重点实验室,江西南昌330004 [2]川北医学院药学院,四川南充637000

出　　处：《中国实验方剂学杂志》2015年第1期158-162,共5页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：国家自然科学基金项目(81460575;81260612);江西省自然科学基金项目(20132BAB205092);江西省青年科学基金项目(20122BAB215039)

摘　　要：目的:探讨辛弗林(synephrineon,Syn)对豚鼠离体气管平滑肌的作用及其机制。方法:采用离体气管恒温灌流的实验方法,取豚鼠气管,在K-H液中制备气管平滑肌环,通过BL-420S生物机能实验系统测定其张力的变化,观察Syn对豚鼠离体气管平滑肌静息张力及对4种致痉剂乙酰胆碱(Ach),组胺(His),Ca Cl2,β受体阻断剂普萘洛尔(Pro)诱导气管平滑肌收缩的影响。结果:Syn可剂量依赖性的舒张气管平滑肌,PD2值为(2.22±0.14);Syn 2个浓度(0.021 2,0.042 4 mmol·L-1)使Ach和His诱发的豚鼠气管平滑肌收缩的最大效应降低,且0.042 4 mmol·L-1的Syn溶液对其均有显著性差异(P<0.05);Syn溶液对Ca Cl2的诱发豚鼠气管平滑收缩效应降低但无显著性差异。Syn 3个浓度(0.010 6,0.021 2,0.042 4 mmol·L-1)对Ach引起的气管平滑肌细胞内钙的释放有显著的抑制作用(P<0.05,P<0.01),且具有剂量依赖关系;加入Pro孵育后,再累积加入Syn,对Ach引起的气管收缩略有降低,与Pro组比较,无显著性差异。结论:Syn可能是通过抑制M受体和组胺受体、兴奋β受体、拮抗内钙的释放从而舒张气管平滑肌。Objective： To investigate the effects and the mechanisms of synephrine （Syn） on the contraction of isolated guinea-pig tracheal smooth muscle. Method： The guinea-pig tracheal smooth muscle was prepared by isolated tracheal thermostatic perfusion experimental method and isolated in the K-H solution. The tension of Syn on the isolated tracheal smooth muscle and the effect of Syn on tracheal smooth muscle contraction incubation by four antispasmodic agents including acetylcholine （ Ach）, histamine （ His）, CaC12 and β-receptor blocking agent （propranolol） were measured by BL-420S biological function experiment systems. Result： Syn produced a dose-dependent relaxant effect on the isolated guinea-pig tracheal smooth muscle. The value of PD2 was 2.22±0. 14. Syn （0. 021 2, 0. 042 4 mmol·L-1 ） could reduce the maximum of guinea-pig tracheal smooth muscle contraction induced by Ach and His, and Syn at 0. 042 4 mmol·L-1 had significant effect （P 〈 0.05 ）. Syn also could reduce the value of guinea-pig tracheal smooth muscle contraction induced by CaC12 with no significant differences. Three dosages of Syn （0. 010 6, 0. 021 2, 0. 042 4 mmol·L-1） could inhibit the release of internal calcium of tracheal smooth muscle cell induced by Ach significantly in a dose-dependent manner （P 〈 0.05, P 〈 0.01 ）. The propranolol and Syn had no significant reducing effect on tracheal tension. Conclusion： Syn couldrelax tracheal smooth muscle by acting on M-receptor and histamine receptor, excit β-receptor and inhibiting the release of internal calcium.

关 键 词：辛弗林 气管平滑肌 舒张 

分 类 号：R285.5[医药卫生—中药学]