DNA与CdS-NH_2纳米粒子的相互作用研究  

The Study on the Interactions between DNA and Cd S-NH_2 Nanoparticles

作　　者：申源[1] 叶树虹[1] 汪莉[1] 陈受惠[1] 

机构地区：[1]江西师范大学化学化工学院,江西南昌330022

出　　处：《江西师范大学学报（自然科学版）》2014年第6期624-631,共8页Journal of Jiangxi Normal University(Natural Science Edition)

基　　金：国家自然科学基金(2105005);江西省教育厅科技课题(GJJ10389);江西师范大学青年成长基金(5487)资助项目

摘　　要：采用荧光光谱、紫外光谱(UV-vis)、圆二色谱(CD)、透射电镜(TEM)、原子力显微镜(AFM)、琼脂糖凝胶电泳等技术研究Cd S-NH2-EcoRI复合物与DNA的相互作用.研究发现:Cd S-NH2纳米粒子与p BR322DNA结合后会延迟EcoRI的酶切反应.DNA的曲率和纳米粒子的粒径都是影响结合作用的因素,曲率较大的环状DNA比线性DNA能更好地与纳米粒子结合,小粒径的Cd S-NH2纳米粒子则更易结合到DNA上.并研究了DNA与Cd S-NH2纳米粒子之间的作用机理.In this work,the interaction between DNA and CdS-NH2-EcoRI composites was investigated by infrared spectroscopy,UV-vis spectroscopy,fluorescence spectroscopy,CD spectroscopy,atomic force microscope,agarose gel electrophoresis,etc. It was shown that the restriction enzyme reaction of EcoRI would be retarded after CdS-NH2 nanoparticles combined with pBR322DNA. Both the curvature of DNA and the particle size of nanoparticles are the crucial factors influencing the groove binding. Circular-like DNA with larger curvature is more easily combined with nanoparticles than linear DNA. And CdS-NH2 nanoparticles with smaller size were more easily combined with DNA. The interaction mechanism of DNA and CdS-NH2 nanoparticles was also studied in this work.

关 键 词：CdS-NH2 CdS-NH2-EcoRI复合物 荧光光谱 圆二色谱 原子力显微镜 琼脂糖凝胶电泳 

分 类 号：Q523[生物学—生物化学] TB383.1[一般工业技术—材料科学与工程]